Thermo Scientific™

SureQuant™ Phosphopeptide Suitability Standards

Catalog number: A51746
Thermo Scientific™

SureQuant™ Phosphopeptide Suitability Standards

Catalog number: A51746
The SureQuant Multipathway Phosphopeptide Standard contains an optimized mixture of 131 isotopically labeled phosphopeptides to simultaneously monitor and quantitate phosphorylation signaling. The SureQuant Phosphopeptide Suitability Standard contains a mixture of 20 isotopically labeled phosphopeptides with increasing hydrophobic properties for LC optimization and monitoring of LC-MS/MS system performance. When used together, the standards help ensure optimal instrument performance and sample assessment for phosphorylation events.
 
Catalog Number
A51746
Unit Size
50 µL
Concentration
500 fmol/μL
Price (USD)
Price: 269.00
Online price: 253.65
Your price:
Full specifications
Quantity
DescriptionSureQuant™ Phosphopeptide Suitability Standard (500 fmol/μL)
Concentration500 fmol/μL
Quantity50 μL
Unit Size50 µL
Catalog Number
Unit Size
200 µL
Concentration
100 fmol/μL
Price (USD)
Price: 1,192.00
Online price: 1,166.65
Your price:
Full specifications
Quantity
DescriptionSureQuant™ Multipathway Phosphopeptide Standard (100 fmol/μL)
Concentration100 fmol/μL
Quantity200 μL
Unit Size200 µL
Showing 2 of 2
Catalog NumberSpecificationsUnit SizeConcentrationPrice (USD)Quantity
A51746Full specifications
50 µL500 fmol/μL
Price: 269.00
Online price: 253.65
Your price:
DescriptionSureQuant™ Phosphopeptide Suitability Standard (500 fmol/μL)
Concentration500 fmol/μL
Quantity50 μL
Unit Size50 µL
A51745Full specifications
200 µL100 fmol/μL
Price: 1,192.00
Online price: 1,166.65
Your price:
DescriptionSureQuant™ Multipathway Phosphopeptide Standard (100 fmol/μL)
Concentration100 fmol/μL
Quantity200 μL
Unit Size200 µL
Showing 2 of 2

The specific phosphopeptides in the SureQuant Multipathway Phosphopeptide Standard have been chosen to cover biologically relevant phosphorylation sites for 89 key proteins from seven different signaling pathways including EGFR/HER, RAS-MAPK, PI3K/AKT/mTOR, AMPK, death/apoptosis, and stress (p38/SAPK/JNK). For optimal results, MS sample preparation should include phosphopeptide enrichment, with the standard spiked in before MS sample preparation (1 pmol) or after phosphopeptide enrichment (0.8 pmol). The ability to simultaneously monitor and quantitate these targets in a single sample is a powerful tool for assessment of normal and abnormal signaling pathway activity.

The phosphopeptides selected for the SureQuant Phosphopeptide Suitability Standard represent relevant proteins from key signaling pathways that include serine, threonine, and tyrosine phosphorylation. The 20 phosphopeptides have a wide range of retention times and have been designed for use with phosphopeptide samples to assess system performance and to better enable prediction of peptide retention times to maximize phosphopeptide identifications.

Features include:
• Optimized—validated mixture of 131 HeavyPeptide AQUA Basic phosphorylated peptides (SureQuant Multipathway standard) or 20 HeavyPeptide AQUA Basic phosphopeptides (SureQuant Suitability standard)
Multiplex—suitable for relative quantitation of phosphorylationevents for 89 key proteins in seven cellular signaling pathways (SureQuant Multipathway standard)
Predictive—range of peptides with increasing hydrophobic properties for method optimization and calculation of peptide retention times
Monitoring—excellent QC assay tool for quality assessment of LC and MS instrument performance (SureQuant Suitability standard)
Convenient—provided in a ready-to-use liquid format

Applications:
• Standard for data-dependent acquisition (DDA)
• Standard for targeted MS (e.g., SRM, PRM)
• Optimization of LC parameters
• Identification of total peptide elution window
• Optimization of MS parameters

For optimal results, MS sample preparation should include phosphopeptide enrichment. We recommend use of the EasyPep Maxi MS Sample Prep Kit (Cat. No. A45734) combined with the High Select Phosphopeptide Enrichment Kits (Cat. No. A32992, A32993, or A52283) for sample preparation. Standardized methods and data process templates are provided for streamlined data acquisition and analysis.

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