SuperScript™ IV RT-LAMP Master Mix
SuperScript™ IV RT-LAMP Master Mix
Invitrogen™

SuperScript™ IV RT-LAMP Master Mix

Invitrogen SuperScript IV RT-LAMP Master Mix is a ready-to-use solution optimized for fast, sensitive, and simple RNA target detection in자세히 알아보기
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카탈로그 번호반응 수
A518031000 Reactions
A51801100 Reactions
A51802400 Reactions
카탈로그 번호 A51803
제품 가격(KRW)
2,445,000
온라인 행사
Ends: 31-Mar-2026
2,876,000
할인액 431,000 (15%)
Each
카트에 추가하기
반응 수:
1000 Reactions
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
2,445,000
온라인 행사
Ends: 31-Mar-2026
2,876,000
할인액 431,000 (15%)
Each
카트에 추가하기

Invitrogen SuperScript IV RT-LAMP Master Mix is a ready-to-use solution optimized for fast, sensitive, and simple RNA target detection in loop-mediated isothermal amplification (LAMP) reactions. With this convenient master mix formulation, you can perform isothermal amplification from both RNA or DNA targets in a single tube format. 

Benefits of SuperScript IV RT-LAMP Master Mix
• RNA and DNA target detection using Bst DNA polymerase for isothermal amplification in as little as five minutes
• Robust and specific cDNA synthesis using SuperScript IV Reverse Transcriptase
• Enhanced specificity and sensitivity with buffer formulation and RNaseOUT recombinant ribonuclease inhibitor
• Compatible with real-time and endpoint detection—includes SYTO 9 dye for real-time detection
• Flexible design provides several options for evaluating results, including real-time and endpoint detection methods

SuperScript IV RT-LAMP Master Mix includes an evolved Bst DNA polymerase mutant with a fast reaction speed and increased sensitivity and tolerance to inhibitors, as well as SuperScript IV Reverse Transcriptase with increased processivity, thermostability, and inhibitor resistance. To prevent RNA degradation by carry-over RNases, the master mix includes the potent, noncompetitive RNaseOUT Recombinant Ribonuclease Inhibitor. Our proprietary reaction buffer composition is optimized to increase specificity by delaying no-template control (NTC) amplification time.

사양
농도2X
검출 방법Real-time and End-point Detection
라벨 또는 염료 포함SYTO 9 Green Fluorescent Nucleic Acid Stain
반응 수1000 Reactions
최적 반응 온도65°C
반응 시간5 to 15 minutes
역전사 효소SuperScript IV
배송 조건Dry Ice
용도(애플리케이션)LAMP, RT-LAMP
중합효소Bst DNA Polymerase
제품 유형Master Mix
수량1000 reactions, 10 x 1.25 mL
Unit SizeEach
구성 및 보관

• 2X SuperScript IV RT-LAMP Master Mix (10 x 1.25 mL)
• SYTO Green Fluorescent Nucleic Acid Stain (30 μL)

Store at –20°C and protect from light.

자주 묻는 질문(FAQ)

Is it possible to combine an (RT-)LAMP reaction with colorimetric detection methods using SuperScript IV RT-LAMP Master Mix?

Yes, LAMP can be combined with colorimetric detection using calcein. We recommend adding calcein and MnCl₂ to the reaction mixture at final concentrations of 80 µM and 0.25 mM, respectively. Prior to amplification, calcein binds to Mn²⁺ ions, giving the mixture an orange color. During amplification, Mn²⁺ ions form a complex with pyrophosphate, releasing calcein and causing the reaction mixture to change to a green or yellowish-green color, indicating successful amplification.