Invitrogen™

TrueTag™ Donor DNA Kit, GFP stem

Catalog number: A53812
Have Questions?
Invitrogen™

TrueTag™ Donor DNA Kit, GFP stem

Catalog number: A53812
Have Questions?
Catalog Number
A53812
Unit Size
10 reactions
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A5381210 reactions
-
Product Overview
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The Invitrogen TrueTag Donor DNA Kit, GFP stem, provides a rapid solution to drive high-efficiency tagging (up to 100% edited cells) of silent or differentially expressed genes with transfection-ready donor DNA.

Benefits of the TrueTag Donor DNA stem kits include:
• One-step PCR amplification to generate donor templates—no cloning required
• Generate transfection-ready DNA donors in hours instead of days
• Save up to three months with enrichment compared to single cell isolation and characterization
• Enrich up to 100% GFP-tagged cells

The TrueTag Donor DNA Kit, GFP stem, provides everything you need to produce a high-quality DNA donor to tag your gene with a selection marker and GFP—simply provide two locus-specific primers to add homology arms and PCR-amplify the donor. Each kit is designed to enable you to:
• Enrich tagged cells even when the target protein is not being expressed
• Choose between a fusion or co-expressed fluorescent tag
• Choose from puromycin or blasticidin selection markers—whatever best suits your cell model
• Easily remove antibiotic resistance markers with Cre/Lox following cell enrichment

Included in the kit
• Four linear donor templates: C-terminus/puromycin resistance + 2A, C-terminus/blasticidin resistance + 2A, C-terminus/puromycin resistance, C-terminus/blasticidin resistance
• Phusion Flash High-Fidelity PCR Master Mix
• PCR cleanup columns and buffers
• Positive control primers to tag human glial fibrillary acidic protein (GFAP)

TrueDesign Genome Editor software to rapidly design gene-specific reagents
Target-specific gene editing reagents are required for proper knock-in of your fluorescent tag. Identify your target gene transcript of interest and the TrueDesign Genome Editor web-based software does the work for you:
1. Displays your target gene transcript
2. Designates your insertion site
3. Designs and enables easy ordering of all required components not included in the TrueTag kit:
    • CRISPR guide RNA or TALENs designed adjacent to N- or C-terminus
    • Oligos to PCR-amplify your donor and add appropriate homology arms
    • Sequencing primers for assessment of CRISPR-Cas9 cleavage efficiency
    • Verification primers to validate proper insertion of the TrueTag DNA Donor

Also required and available for purchase separately or as part of TrueDesign workflow:
TrueCut Cas9 Protein v2, TrueCut HiFi Cas9 Protein, or GeneArt CRISPR mRNA
CRISPRMAX Transfection Reagent or MessengerMAX Transfection Reagent

For Research Use Only. Not for use in diagnostic procedures

Specifications

For Use With (Application)
Cellular Imaging
No. of Reactions
10 reactions

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