CTS™ Viral Production Cells

CTS Viral Production Cells are derived from the human HEK293 cell line and are a core component of the CTS詳細を見る

製品番号（カタログ番号）	数量
A53956	1.5 mL

製品番号（カタログ番号） A53956

価格（JPY）

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1.5 mL

CTS Viral Production Cells are derived from the human HEK293 cell line and are a core component of the CTS LV-MAX lentiviral production system to produce maximal lentiviral titers. The cells allow high density, serum-free suspension culture with minimal cell clumping in CTS LV-MAX Production Medium. They are specifically validated for lentiviral vector production for the purpose of cell and gene therapy applications, but may be suitable for the production of other viral vectors where the absence of SV40 large T antigen in HEK293 cells is desired. These cGMP-banked cells have gone through a documented validation process to ensure that they can be transfected with multiple plasmids and that viable viral particles can be produced.

Features of the CTS Viral Production Cells include:

Fully-documented cGMP-banked cell line (HEK293F-derived) with detailed cell line lineage history
Extensive quality testing based on the US FDA and European Medicines Agency (EMA) guidances for therapeutic use
Flexible licensing options without royalties

Please visit www.thermofisher.com/celllicenserequest for more information and access.

細胞ベース、遺伝子ベース、または組織ベースの製品の研究での用途または製造。注意：ヒトまたは動物への直接投与は意図されていません。

使用対象（アプリケーション）Cell & Gene Therapy Research, Development, and Manufacturing

フォーマットCryopreserved vial

製造品質21 CFR Parts 210 and 211

推奨培地Gibco™ CTS™ Viral Production Medium

セル数See Certificate of Analysis

製品タイプViral Production Cell Line

数量1.5 mL

品質保持期間10 years from date of manufacture

出荷条件Dry ice or vapor phase liquid nitrogen

試験済みLV Production

細胞株293F

細胞タイプ腎臓（胚性）

Culture Type懸濁細胞培養

Unit SizeEach

組成および保存条件

1 x 10⁷ cells in a 2-mL vial. Store in vapor phase liquid nitrogen.

よくあるご質問（FAQ）

Are CTS Viral Production Cells 293T-derived?

No, CTS Viral Production Cells are HEK 293F suspension cells that have been adapted to CTS LV-MAX Production Medium. They do not express the SV40 large T antigen.

How do I bank CTS Viral Production Cells?

CTS Viral Production Cells can be frozen directly in cryogenic medium consisting of 90% CTS LV-MAX Production Medium plus 10% DMSO. Here is the protocol:

- Bank cells at passage 3 when cell density reaches ˜3.0-4.5 x 10E6 viable cells/mL at >95% viability.
- Centrifuge the cells at 100 x g (low speed) for 5 mins to pellet. Discard the spent medium.
- Prepare freezing medium: e.g., 10 mL of freezing medium is 9 mL of CTS LV-MAX Production Medium + 1 mL DMSO; mix well and sterilize by 0.2 µm filtration.
- Resuspend the cell pellet in the prepared freezing medium.
- Dilute the cells to a final density of 10 x10E6 viable cells/mL and aliquot 1 mL per cryovial.
- Freeze the cells at -80 degrees C for 1 day.
- Transfer frozen vials to liquid nitrogen for long-term storage.

Can I use CTS Viral Production Cells (Cat. No. A53956) with CTS AAV MAX products?

CTS Viral Production Cells 2.0 are specifically designed to work with CTS AAV MAX products to provide maximal adeno-associated virus (AAV) titers and optimal performance. CTS Viral Production Cells can be used but we don't recommend doing this as it will reduce system performance.