Q: What is the turnaround time (TAT) for running 48 samples on the Ion GX5 chip?

The turnaround time (TAT) for running 48 samples on the Ion GX5 chip is 18 hours. Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center .

Q: Using the Ion AmpliSeq Polyploidy Panel Kit with Ion GX5 Chips (384 Samples) (Cat. No. GX55689), can I run samples from Ion ReproSeq PGT-A library and Ion AmpliSeq Polyploidy Panel library in the same lane of the Ion GX5 chip?

Samples from Ion ReproSeq PGT-A and Ion AmpliSeq Polyploidy Panel libraries must be run in separate lanes of the Ion GX5 chip. Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center .

Q: How many samples in total can I run with the Ion AmpliSeq Polyploidy Panel Kit with Ion GX5 Chips (384 Samples) (Cat. No. GX55689)?

The kit is sufficient for running up to 384 samples since 2 chips are included in the kit with each chip having 4 lanes where one can run up to 48 samples per lane. Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center .

Q: How many samples in total can I run with Ion AmpliSeq Polyploidy Panel Kit with Ion 520 Chips (80 Samples) (Cat. No. GS45691)?

The kit is sufficient for running up to 80 samples since 4 chips are included in the kit and each chip can run up to 20 samples. Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center .

Q: How should I store the Ion AmpliSeq Polyploidy Panel?

Ion AmpliSeq Polyploidy Panel should be stored at -10 to -30 degrees C. Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center .

Q: What version of software is compatible with the Ion AmpliSeq Polyploidy Panel on the Genexus instrument?

The Ion AmpliSeq Polyploidy Panel plugin requires Genexus Software v6.8 or later. Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center .

Q: Using the Ion AmpliSeq Polyploidy Panel, how many samples can be multiplexed on Ion 520 and 530 chips?

With the Ion 530 chip, you can run up to 60 samples per chip With the Ion 520 chip, you can run up to 20 samples per chip Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center .

Q: How many samples can be run using the Ion AmpliSeq Polyploidy Panel?

The Ion AmpliSeq Polyploidy Panel is a single pool design for germline SNP detection and supplies sufficient reagents for running up to 96 samples. Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center .

Q: How much of each sample is needed for the Ion AmpliSeq Polyploidy Panel?

We recommend using at least 3 cells from embryo biopsy samples, keeping in mind that data quality increases with input. We have tested up to 10 cells in house but expect good tolerance at higher levels. If using gDNA, we recommend using at least 75 pg. Please note that dilutions of gDNA are prone to loss to plastics. Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center .

Question 1

How many samples in total can I run with Ion AmpliSeq Polyploidy Panel Kit with Ion 530 Chips (480 Samples) (Cat. No. GS45690)?

Accepted Answer

The kit is sufficient for running up to 480 samples since 8 chips are included in the kit and each chip can run up to 60 samples.<

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Question 2

What is the turnaround time (TAT) for running 48 samples on the Ion GX5 chip?

Accepted Answer

The turnaround time (TAT) for running 48 samples on the Ion GX5 chip is 18 hours.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Question 3

Using the Ion AmpliSeq Polyploidy Panel Kit with Ion GX5 Chips (384 Samples) (Cat. No. GX55689), can I run samples from Ion ReproSeq PGT-A library and Ion AmpliSeq Polyploidy Panel library in the same lane of the Ion GX5 chip?

Accepted Answer

Samples from Ion ReproSeq PGT-A and Ion AmpliSeq Polyploidy Panel libraries must be run in separate lanes of the Ion GX5 chip.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Question 4

How many samples in total can I run with the Ion AmpliSeq Polyploidy Panel Kit with Ion GX5 Chips (384 Samples) (Cat. No. GX55689)?

Accepted Answer

The kit is sufficient for running up to 384 samples since 2 chips are included in the kit with each chip having 4 lanes where one can run up to 48 samples per lane.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Question 5

How many samples in total can I run with Ion AmpliSeq Polyploidy Panel Kit with Ion 520 Chips (80 Samples) (Cat. No. GS45691)?

Accepted Answer

The kit is sufficient for running up to 80 samples since 4 chips are included in the kit and each chip can run up to 20 samples.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Question 6

How should I store the Ion AmpliSeq Polyploidy Panel?

Accepted Answer

Ion AmpliSeq Polyploidy Panel should be stored at -10 to -30 degrees C.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Question 7

What version of software is compatible with the Ion AmpliSeq Polyploidy Panel on the Genexus instrument?

Accepted Answer

The Ion AmpliSeq Polyploidy Panel plugin requires Genexus Software v6.8 or later.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Question 8

Using the Ion AmpliSeq Polyploidy Panel, how many samples can be multiplexed on Ion 520 and 530 chips?

Accepted Answer

With the Ion 530 chip, you can run up to 60 samples per chip
With the Ion 520 chip, you can run up to 20 samples per chip

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Question 9

How many samples can be run using the Ion AmpliSeq Polyploidy Panel?

Accepted Answer

The Ion AmpliSeq Polyploidy Panel is a single pool design for germline SNP detection and supplies sufficient reagents for running up to 96 samples.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Question 10

How much of each sample is needed for the Ion AmpliSeq Polyploidy Panel?

Accepted Answer

We recommend using at least 3 cells from embryo biopsy samples, keeping in mind that data quality increases with input. We have tested up to 10 cells in house but expect good tolerance at higher levels. If using gDNA, we recommend using at least 75 pg. Please note that dilutions of gDNA are prone to loss to plastics.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Question 11

What controls can be used with the Ion AmpliSeq Polyploidy Panel?

Accepted Answer

The Control DNA (from CEPH individual 1347-02) (Cat. No. 403062) can be used with the Ion AmpliSeq Polyploidy Panel.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Question 12

What regions are targeted with the Ion AmpliSeq Polyploidy Panel?

Accepted Answer

The Ion AmpliSeq Polyploidy Panel is a single pool for comprehensive coverage of >500 SNP sites. It comprises 74 microhaplotype amplicons for 222 SNP sites used in human identification and 368 single-SNP amplicons for population-wide representation based on minor allele frequencies in the 1000 Genomes Project.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Question 13

Is there an automated option to prep a library from the Ion AmpliSeq Polyploidy Panel?

Accepted Answer

No, the library must be created manually.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Question 14

What is the Ion AmpliSeq Polyploidy Panel?

Accepted Answer

The Ion AmpliSeq Polyploidy Panel is now available as an add-on to Ion ReproSeq PGS kits. The Ion AmpliSeq Polyploidy Panel is designed to deliver genetic insights on triploidy, maternal DNA contamination, and sibling QC through targeted sequencing of single-nucleotide polymorphisms (SNPs). The panel is compatible with both the Ion GeneStudio and Genexus systems.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Ion AmpliSeq™ Polyploidy Panel - FAQs