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TrueMark™ STI Amplification Control

Applied Biosystems™

TrueMark™ STI Amplification Control

The TrueMark STI Amplification Control contains a linearized multi-target plasmid with the following targets: Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium,Read more

Catalog Number	Quantity
A57009	25 μL

Catalog number A57009

The TrueMark STI Amplification Control contains a linearized multi-target plasmid with the following targets: Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Trichomonas vaginalis, and the human RNaseP gene.

The amplification control has a concentration of 1 x 10⁵ copies/μL. It can be used as a positive control for STI panel-specific amplification or as a stand-alone sample to verify assay performance and to help with troubleshooting.

Detection MethodPrimer-probe

For Use With (Application)Pathogen Detection

Frequently asked questions (FAQs)

Is there any cross-reactivity reported for the TrueMark STI Select Panel, Combo Kit?

Cross-reactivity was observed with the following organisms when using the TrueMark STI Select Panel, Combo Kit:
Organism Pentatrichomonas hominis -> Cross-reactive target: Trichomonas vaginalis
Organism Trichomonas tenax -> Cross-reactive target: Trichomonas vaginalis

What are the reporter dyes, targets, and quenchers used in the TrueMark STI Select Panel, Combo Kit?

Please refer to Table 1 on page 4 of the user guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0028493-TrueMarkSTISelectPanel-UG-RUO-EN.pdf) for dyes, targets, and quenchers used in the TrueMark STI Select Panel, Combo Kit.

What specimen types can be used with the TrueMark STI Select Panel, Combo Kit?

Vaginal and genital swabs can be used with the TrueMark STI Select Panel, Combo Kit.

What is the approximate workflow time for running the TrueMark STI Select Panel, Combo Kit assay starting from sample preparation?

It takes approximately 3 hrs for running the TrueMark STI Select Panel, Combo Kit assay, starting from sample preparation to obtaining the Ct value.

With the TrueMark STI Select Panel, Combo Kit and TrueMark STI Amplification Control, how long can I hold the reagents/reaction before starting the procedure?

TrueMark STI Select Panel: Once thawed, the assay is stable for up to 8 hrs at 2-8 degrees C. Do not exceed 5 freeze-thaws.
TrueMark STI Amplification Control: Once thawed, the amplification control should be used immediately and returned to -30 to -10 degrees C. Do not exceed 5 freeze-thaws.
Assembled RT‑PCR reaction plate: The assembled RT-PCR reaction plate is stable for up to 60 mins at 2-8 degrees C.

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