Invitrogen CellMask Deep Red Actin Tracking Stain fluorescently labels polymerized/filamentous actin (F-actin) in live or fixed cells. It is designed to readily permeate live cells, thus providing more uniform and bright labeling. CellMask Deep Red Actin Tracking Stain can be used to track F-actin in live cells for 24 hours or more. Live cells stained with CellMask Deep Red Actin Tracking Stain can be fixed, or it can be used with fixed cells. And the stain can be used for multiplexing with antibodies in immunofluorecence (IF), immunocytochemistry (ICC), and immunohistochemistry (IHC) protocols.
Features of Cell Mask Deep Red Actin Tracking Stain include:
• Stains F-actin in live or fixed cells for fluorescence imaging
• Can be multiplexed with antibodies in IF/ICC/IHC experiments
• Fluorescence maxima: Ex 652 nm/Em 669 nm; can be detected with CY5/Deep Red filterSelection guide for cellular cytoskeleton staining probes
CellMask actin tracking stains are available in three colors: green (Cat. No. A57246) detected with a FITC/GFP filter (Ex 503 nm/Em 512 nm), orange (Cat. No. A57247) detected with a TRITC/RFP filter (Ex 545 nm/Em 570 nm), and red (Cat. No. A57248) detected with a CY5/Deep Red filter (Ex 652 nm/Em 669 nm). Excitation and emission spectra of all three stains are provided below.
CellMask actin tracking stains detect F-actin with a targeting structure that closely resembles jasplakinolide. They do not label globular actin (G-actin) or monomer actin (M-actin). CellMask actin tracking stains easily pass through live cell membranes and are retained within live cells after loading. As demonstrated in the figures below, incubation with CellMask actin tracking stains has no detectable effect on viability of various cell lines after 24 hours of incubation.
CellMask actin tracking stains detect F-actin in live cells or tissue, which can then be fixed. In addition, CellMask actin tracking stains can be used to detect F-Actin in paraformaldehyde fixed cells or tissue (freshly fixed or cryo frozen only) and can be multiplexed with antibody detection. CellMask actin tracking stains can also be used in 3D cell culture for 3D imaging.