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Invitrogen™

MegaScript™ T7 Transcription Kit Plus

Catalog number: A57622
Invitrogen™

MegaScript™ T7 Transcription Kit Plus

Catalog number: A57622
Catalog Number
A57622
Unit Size
50 reactions
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
1000 reactions
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A5762250 reactions
-
A576231000 reactions
-
Product Overview
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The MegaScript T7 Transcription Kit Plus contains all the reagents and buffers necessary for in vitro synthesis of large amounts of RNA. Each 20-μL reaction yields an average of 100 μg of RNA, while the scaled-up protocol yields up to 1 mg of RNA. This kit is suitable for full- or partially-modified-nucleotide substitution to produce modified RNA. It is also suitable for co-transcriptional capping with cap analogs to make capped RNA that can be translated into protein.

Features of the MegaScript T7 Transcription Kit Plus include:
• Optimized to achieve high mRNA yields (>5 mg/mL)
• Flexible—allows use of modified nucleotides and/or cap analogs such as ARCA or CleanCap
• Includes GFP control template
• Made up of high quality reagents (TheraPure)
• Available in two sizes: 50 and 1000 reactions

The MegaScript T7 Transcription Kit Plus requires a linearized DNA template with a T7 RNA polymerase promoter to create >5 mg/mL of RNA. MEGAscript kits are intended for the synthesis of large amounts of unlabeled or low-specific-activity RNA for a variety of uses, including transfections, preclinical mRNA therapeutic mRNA studies, microinjections, in vitro translation, and RNA structure and function analysis. Note that this kit does not create capped RNA, which is necessary for translational studies. MEGAscript kits are not recommended for synthesis of high-specific-activity probes.

For Research Use Only.

Specifications

Cell Type
HEK293, 293, HeLa, CHO-K1, CHO, Jurkat, Primary Human T Cells, Primary Cells, Stem Cells, iPSC, NIH-3T3, HepG2, A549, HUVEC
Final Product Type
In vitro Transcribed RNA
Format
Kit
Isolation Technology
Silica Spin Column
Purification Target
In vitro Transcribed RNA
Sample Type
In vitro Transcribed RNA, Total RNA
No. of Reactions
50 Reactions

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