A novel anti-HER2/EGFR bispecific antibody-drug conjugate demonstrates promising antitumor efficacy and overcomes resistance to HER2- or EGFR-targeted ADCs.
AuthorsHuang H,Zhou Y,Shang C,Zhang Y,Shen Y
JournalInvestigational new drugs
PubMed ID39982632
HER2 and EGFR are frequently co-expressed in various tumors. While antibody-drug conjugates (ADCs) targeting HER2, such as T-DM1 and T-Dxd, have shown remarkable antitumor effects in clinical responses, their effectiveness is constrained by drug resistance. EGFR amplification or high expression is one of the factors that lead to resistance against ... More
Simultaneous analysis of antigen-specific B and T cells after SARS-CoV-2 infection and vaccination.
AuthorsNewell KL,Waldran MJ,Thomas SJ,Endy TP,Waickman AT
JournalCytometry. Part A : the journal of the International Society for Analytical Cytology
PubMed ID35468250
Conventional methods for quantifying and phenotyping antigen-specific lymphocytes can rapidly deplete irreplaceable specimens. This is due to the fact that antigen-specific T and B cells have historically been analyzed in independent assays each requiring millions of cells. A technique that facilitates the simultaneous detection of antigen-specific T and B cells ... More
Structural basis for potent neutralization of human respirovirus type 3 by protective single-domain camelid antibodies.
Respirovirus 3 is a leading cause of severe acute respiratory infections in vulnerable human populations. Entry into host cells is facilitated by the attachment glycoprotein and the fusion glycoprotein (F). Because of its crucial role, F represents an attractive therapeutic target. Here, we identify 13 F-directed heavy-chain-only antibody fragments that ... More
Purification and quantitation of bacteriophage M13 using desalting spin columns and digital PCR.
Separation of small molecules such as biotinylated baits from solutions of filamentous bacteriophage is achieved generally through polyethylene glycol precipitation of the phage and centrifugation prior to affinity selection or panning. This method is laborious and time-consuming and is accompanied frequently by significant loss of virions, especially when performed at ... More
Efficient charge transport via limited π-π interactions between naphthyl carbon atoms in a metal-organic framework.
A novel metal-organic framework based on naphthalenediimide derivatives was synthesized, featuring ONDI(2-) ligands arranged in a slipped stacking pattern that forms H-aggregates with limited π-π interactions between two pairs of adjacent naphthyl carbon atoms. The compound demonstrates typical semiconductive behavior, with a conductivity of 3.1 × 10(-7) S cm(-1) at ... More
The nutrient-sensing Rag-GTPase complex in B cells controls humoral immunity via TFEB/TFE3-dependent mitochondrial fitness.
Germinal center (GC) formation, which is an integrant part of humoral immunity, involves energy-consuming metabolic reprogramming. Rag-GTPases are known to signal amino acid availability to cellular pathways that regulate nutrient distribution such as the mechanistic target of rapamycin complex 1 (mTORC1) pathway and the transcription factors TFEB and TFE3. However, ... More
Rag-GTPase-TFEB/TFE3 axis controls B cell mitochondrial fitness and humoral immunity independent of mTORC1.
During the humoral immune response, B cells undergo rapid metabolic reprogramming with a high demand for nutrients, which are vital to sustain the formation of the germinal centers (GCs). Rag-GTPases sense amino acid availability to modulate the mechanistic target of rapamycin complex 1 (mTORC1) pathway and suppress transcription factor EB ... More