MagMAX Pro HT NoSpin Plasmid MiniPrep Kit, 96 reactions - FAQs

View additional product information for MagMAX™ Pro HT NoSpin Plasmid MiniPrep Kit - FAQs (A58312, A58309, A58310, A58311)

5 product FAQs found

Can I grow an overnight bacterial culture directly in the KingFisher 96-well plate when using the MagMAX Pro HT NoSpin Plasmid MiniPrep Kit?

Yes, the KingFisher 96-well plate can be used for overnight bacterial culture growth with the MagMAX Pro HT NoSpin Plasmid MiniPrep Kit. However, growth conditions depend on factors such as media type, host strain, plasmid copy number, and plasmid size. For best results, optimize orbital shaker settings and monitor OD600 to minimize well-to-well variation and ensure consistent plasmid yield.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

How does plasmid copy number affect yields with the MagMAX™ Pro HT NoSpin Plasmid MiniPrep Kit?

High-copy plasmids yield higher amounts of DNA and are ideal for most downstream applications. Low-copy plasmids can be extracted, but yields may be lower and may not be sufficient for all transfection protocols. For low-copy plasmids, optimizing culture conditions, increasing culture volume, or performing multiple elution steps may help improve yield.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Which bacterial strains are compatible with the MagMAX Pro HT NoSpin Plasmid MiniPrep Kit?

The MagMAX Pro HT NoSpin Plasmid MiniPrep Kit is compatible with K12 E. coli derivatives, including DH5alpha, TOP10, and DH10B strains. Plasmid yield may vary with low-copy origins, strong host dependencies, or high genomic integration tendencies.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Should I be concerned about bead clumps in the BactBind Beads or PlasmidPure Beads bottles before use?

No, bead settling during transit is normal. This does not affect performance. We recommend vortexing well, before use.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What should I do if there are precipitates in the Lysis Buffer within the MagMAX Pro HT NoSpin Plasmid MiniPrep Kit?

We recommend warming the buffer at 30-37 degrees C for 30 min, and then inverting to mix until the precipitates dissolve.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.