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TMTpro 18plex Label Reagents in stabilized solution, 96-well plate, 4 x 50 μg - FAQs

TMTpro 18plex Label Reagents in stabilized solution, 96-well plate, 4 x 50 μg - FAQs

View additional product information for TMTpro 18-plex, TMTpro-134C, and TMTpro-135N Label Reagents - FAQs (A58335, A52047, A52046, A52048, A52045)

8 product FAQs found

How can I tell if the buffer in which my peptides are resuspended are compatible with TMT and TMTpro labels?

TMT and TMTpro labels are not compatible with buffers containing primary amines. After protein digestion, peptides need to be cleaned up and dried down. Prior to labeling, we do recommend resuspending the peptides in 10 µL of either 100 mM TEAB (pH 8.5) or 100 mM HEPES (pH 8.0).

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Is the TMT stabilizing solution compatible with subsequent MS steps?

For further steps after TMT labelling, the stabilizing solution can be considered as a 33% DMSO solution. If subsequent excess TMT removal will be performed using EasyPep solid-phase extraction (SPE), the DMSO solution does not need to be diluted. On the other hand, the use of any reverse phase chromatography method (i.e., Cat. No. 84868) might need dilution of the sample to ensure that the DMSO concentration is tolerated by the resin. Alternatively, the peptide mix can be dried down using Speed Vac and then resuspended in the corresponding buffer for a particular column (Reverse Phase or Desalting).

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

With TMT 10plex, TMT 11plex, TMTpro 16plex, and TMTpro 18plex 96-well plates, how much protein can be used per TMT or TMTpro reaction?

TMT and TMTpro label reagents in 96-well plates are optimized to work with 1 to 10 µg of protein digest.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Can the sealing foil be partially removed with TMT 10plex, TMT 11plex, TMTpro 16plex, and TMTpro 18plex 96-well plates?

Yes, the sealing foil can be partially peeled to access individual sets of TMT or TMTpro labels. Alternatively, each of the wells can be perforated to transfer the TMT/TMTpro labels to a different tube, if needed.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

How do I determine the correct orientation of the TMT 10plex, TMT 11plex, TMTpro 16plex, and TMTpro 18plex 96-well plates?

All the plates are barcoded in the long lower edge, helping to recognize correct plate orientation, plus the plate has a notch on the left corners.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Would the TMT and TMTpro labels be affected if the TMT 10plex, TMT 11plex, TMTpro 16plex, and TMTpro 18plex 96-well plates are going through several freeze-thaw cycles?

The TMT and TMTpro labels from TMT 10plex, TMT 11plex, TMTpro 16plex and TMTpro 18plex 96-well plates are stable after 9 freeze-thaw cycles, making it possible to use each of the experiment sets at different times.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

With TMT 10plex, TMT 11plex, TMTpro 16plex, and TMTpro 18plex 96-well plates, can I run each of the experiment sets in different runs or should I use the plate all at once?

The entire plate can be run at one time or each experiment set can be run at a different time, using a high-throughput automation platform.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

How many sets of experiments can I run with TMTpro 18plex Label Reagents ​in stabilized solution, ​96-well plate, 4 x 50 ug​ (Cat. No. A58335)?

TMTpro 18plex 96-well plate (Cat. No. A58335) contains 4 independent sets of experiments, each of them occupying three columns with rows A and H empty. Please see the user guide for further plate layout details.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.