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Applied Biosystems™

TaqPath™ DuraPlex™ 1-Step RT-qPCR Master Mix

Catalog number: A58667
Applied Biosystems™

TaqPath™ DuraPlex™ 1-Step RT-qPCR Master Mix

Catalog number: A58667
Catalog Number
A58667
Unit Size
5 x 1 mL
Price (USD)
Price: 2,805.00
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
0.5 mL
Price (USD)
Price: 285.00
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
10 mL
Price (USD)
Price: 5,186.00
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A586675 x 1 mL
Price: 2,805.00
Your price:
-
A586660.5 mL
Price: 285.00
Your price:
-
A5866810 mL
Price: 5,186.00
Your price:
-
Product Overview
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Applied Biosystems TaqPath DuraPlex 1-Step RT-qPCR Master Mix is a benchtop-stable, single-tube master mix optimized for rapid, sensitive, and reproducible detection of viral and bacterial pathogens even in the presence of PCR inhibitors. This automation-friendly master mix is ideally suited for testing and developing high-throughput molecular diagnostics, as well as for gene expression analysis for high-throughput biologics screening. The 4X concentrated formulation can multiplex six targets in a single well and has intuitive user handling.

TaqPath DuraPlex 1-Step RT-qPCR Master Mix is available in two formulations:
With ROX dye as a passive reference—recommended for multiplex assays with up to five targets in a single well*
Without ROX dye as a passive reference—recommended for multiplex assays with up to six targets in a single well
*Not recommended for use with assay utilizing JUN (or similar emission wavelength) dye.

Benefits of TaqPath DuraPlex 1-Step RT-qPCR Master Mix
• Enable workflow automation with benchtop-stability of fully assembled reactions for a full workday
• Optimize your assay for high-order multiplexing of up to six targets in a single reaction
• Improve handling, in-use stability, and freeze-thaw conditions
• Increase confidence in detecting both RNA and DNA targets in samples containing challenging PCR inhibitors
• Ensure reproducible results with consistent lot-to-lot performance

Support workflow automation
TaqPath DuraPlex 1-Step RT-qPCR Master Mix enables a full workday (over eight hours) of stability of completely assembled reactions (master mix, sample, and assay), helping enable the use of liquid handling and other automation equipment. This fast-cycling master mix can be used effectively for both RNA and DNA detection of bacterial, viral, and fungal targets. The figure below shows consistent sensitivity with a multiplex assay tested at 0 and 8 hours compared to other manufacturers.

Optimized for high-order multiplexing
TaqPat DuraPlex 1-Step RT-qPCR Master Mix has been validated with multiplex assays with up to six targets in a single well with the No ROX formulation and up to five targets in a single well with the ROX formulation. It enables accurate detection of both low and high RNA and DNA concentration samples and provides wide dynamic range. The figure below demonstrates the excellent PCR linearity over an 8-log input range in a 5-plex assay.

Improved handling
TaqPath DuraPlex 1-Step RT-qPCR Master Mix has been formulated to improve in-use handling, including a lower viscosity to allow for more effective pipetting, including with automated liquid-handling platforms. The master mix is stable at room temperature (24°C) for up to 1 week and can be stored under refrigeration (4°C) for up to one month after first use It can be used confidently through eight freeze-thaw cycles without loss of performance. The novel formulation helps ensure that the master mix can be adapted to a variety of unique workflow scenarios. The figure below shows consistent performance of reagents stored for one week at 24°C .

Increase confidence
The unique proprietary TaqPath DuraPlex 1-Step RT-qPCR formulation allows robust performance even in the presence of substances that can inhibit PCR, resulting in effective template detection from both purified RNA and DNA, including challenging clinical sample matrices such as VTM, stool, blood, and urine. The figure below shows performance with common PCR inhibitors compared to master mixes from other manufacturers.

Facilitate reproducibility
This General Purpose Reagent (GPR), labeled 'For Laboratory Use', is manufactured in an ISO 13485-certified and FDA-registered facility that adheres to the principles of current good manufacturing practices (cGMP). All lots of TaqPath DuraPlex 1-Step RT-qPCR Master Mix are functionally tested to help ensure lot-to-lot reproducibility for Ct and dynamic range consistency across a wide variety of assays. The figure below shows consistent performance of three lots of master mix.

Compliance package
In an effort to efficiently support your compliance needs, the following frequently requested documents are made available below.
Statement of Quality relating to current good manufacturing practices (cGMP) of TaqPath master mixes
• Detailed Lot Specific Certificate of Analysis (COA) (see below in Documents & Downloads section)
• Supplier evaluation support
--Completed self-assessment
--ISO certificates by manufacturing site
• For EU Reach compliance-related inquiries contact LSGChemicalCompliance@thermofisher.com
• Change Control and Notification Registration

Not finding the compliance document you’re looking for? Technical Support is available to help.

Learn more about multiplexing, TaqPath probes, and how our products can help with your projects ›
 

For Laboratory Use

Specifications

Detection Method
Primer-probe
Form
Liquid
Format
Single-Tube
GC-Rich PCR Performance
Medium
PCR Method
1-step RT-qPCR, Multiplex qPCR
Polymerase
Applied Biosystems™ AmpliTaq™ DNA Polymerase with Hot Start
Reaction Speed
Fast or Standard
Passive Reference Dye
ROX™
Product Line
TaqPath™
Shelf Life
Two years from date of manufacture, with guaranteed minimum shelf life of ≥1 year upon receipt
Shipping Condition
Dry Ice
For Use With (Application)
Virus Detection, Pathogen Detection, Real Time PCR (qPCR), Gene Expression
Concentration
4X
Hot Start
Antibody-based
No. of Reactions
1000 x 20 μL Reactions
Volume
5 mL

Contents & Storage

Store at –15 to –25°C.
Store at room temperature (24°C) for 1 week after first use and 4°C for 1 month after first use.

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    Frequently asked questions (FAQs)

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