Thermo Scientific™

EquiPhi29™ DNA Amplification Kit

Catalog number: A65393
Have Questions?
Thermo Scientific™

EquiPhi29™ DNA Amplification Kit

Catalog number: A65393
Have Questions?
Catalog Number
A65393
Unit Size
100 reactions
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
500 reactions
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A65393100 reactions
-
A65394500 reactions
-
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The Thermo Scientific EquiPhi29 DNA Amplification Kit includes all required components for fast, sensitive, and efficient isothermal DNA amplification, which includes rolling circle amplification (RCA) and multiple displacement amplification – whole genome amplification (MDA-WGA).

Features of the EquiPhi29 DNA Amplification Kit include:
• Fast DNA amplification in less than two hours
• Sensitivity from 1 fg of input DNA
• Maximal yield of 17 µg amplified DNA
• Compatibility with different types of sample input material (e.g., purified DNA, liquid media culture, agar plate colonies)
• Compatibility with various downstream applications such as DNA sequencing (Sanger sequencing, next-generation sequencing), digestion with restriction enzymes, cell-free DNA enrichment, and cell-free protein expression
• All required components for DNA amplification in RCA or MDA-WGA reactions

The kit contains EquPhi29 DNA Polymerase, a proprietary phi29 DNA polymerase mutant that possesses strong strand displacement activity and enables high thermostability, reaction speed, and product yield and low amplification bias. Also included is an optimized reaction buffer, exo-resistant random primer, pyrophosphatase, and dNTPs. The addition of pyrophosphatase to the reaction mixture enhances DNA amplification and facilitates sample handling.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
DNA Amplification Kit
Form
Liquid
For Use With (Application)
RCA, MDA-WGA
Polymerase
EquiPhi29 DNA Polymerase
Quantity
100 Reactions
Research Category
Nucleic Acid Analysis, In Vitro Transcription, Cell-free Protein Expression, In Situ Sequencing, SNP Detection
Format
Kit
Optimal Reaction Temperature
42°C
Shipping Condition
Dry Ice
Reaction Time
Up to 2 hours

Contents & Storage

• EquiPhi29 DNA Polymerase (0.1 mL at 10 U/μL)
• 10X EquiPhi29 DNA Polymerase Reaction Buffer (1 mL)
• 0.1 M DTT (0.25 mL)
• 500 μM Exo-Resistant Random Primer (0.2 mL)
• 10 mM dNTP Mix (0.2 mL)
• 0.1 U/μL Pyrophosphatase, inorganic (0.1 mL)

Store at -15 to -25°C.

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