Invitrogen™

SuperScript™ IV Template Switching RT Master Mix

Catalog number: A65425
Invitrogen™

SuperScript™ IV Template Switching RT Master Mix

Catalog number: A65425
Catalog Number
A65425
Unit Size
5 x 96 reactions
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
48 reactions
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
96 reactions
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A654255 x 96 reactions
-
A6542348 reactions
-
A6542496 reactions
-
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Invitrogen SuperScript IV Template-Switching RT Master Mix is designed for high template switching efficiency in cDNA synthesis reactions. It contains SuperScript IV Reverse Transcriptase, RNase Inhibitor, and all other reverse transcription reaction components in a convenient premixed format. Only RNA template, RT primer, and template switching oligo (TSO) of choice need to be added.

Features of SuperScript IV Template-Switching RT Master Mix include:
• Formulated for high quality full-length cDNA synthesis
• Includes proprietary enhancer to maximize template switching efficiency
• Exceptional sensitivity for easy detection of low abundance targets (as low as single cell or 2 pg total RNA)
• Cell lysis buffer included for direct cDNA synthesis without RNA purification

Procedure overview
To maximize template switching efficiency, SuperScript IV Template-Switching RT Master Mix contains proprietary template switching enhancer and leverages the terminal deoxynucleotidyl transferase (TdT) activity of SuperScript IV Reverse Transcriptase. When the reverse transcriptase reaches the 5’ end of the RNA template, TdT activity adds 1–3 extra nucleotides to the cDNA end, enabling binding of the template switching oligo and incorporation of known sequence at the 3’ end of the resulting cDNA. This cDNA can be used for amplification by PCR, gene expression analysis by real-time PCR, or as a template for 5’RACE, RNA-seq, or second strand cDNA synthesis.This master mix is compatible with a wide input range of total RNA (from 2 pg to 4 µg) and is supplied with lysis buffer to enable direct cDNA synthesis from mammalian cells (1–1,000 cells per sample).

Related products
SuperScript IV Template-Switching RT Master Mix is compatible with various RT primers and self-designed template switching oligos. We offer Template Switching Oligo and Capturing Oligo (dT) Primer, both containing the same adaptor sequence complementary to Preamplification Primer. Alternatively, a full set of reagents for template switching cDNA synthesis and preamplification is available in the SuperScript IV Single Cell/Low-Input cDNA PreAmp Kit.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format
Master Mix
GC-Rich PCR Performance
High
Reaction Speed
30 min.
Technique
Template Switching Reverse Transcription
Concentration
4X
Final Product Type
First-Strand cDNA
No. of Reactions
5 x 96 Reactions
Reagent Type
Reverse Transcription
Starting Material
Cells or RNA

Contents & Storage

Five boxes, each containing:
• 4X SuperScript IV cDNA Synthesis Master Mix (480 μL)
• 10X Lysis Buffer (96 μL)
• Nuclease-free water (1.25 mL)

Store at -15 to -25°C.

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