Search

  • Contact Us
  • Quick Order
CBQCA Plus Protein Quantitation Kit - FAQs

CBQCA Plus Protein Quantitation Kit - FAQs

View additional product information for CBQCA Plus Protein Quantitation Kit - FAQs (A66522)

11 product FAQs found

What is the difference between the CBQCA Plus Protein Quantitation Kit (Cat. No. A66522) and the CBQCA Protein Quantitation Kit (Cat. No. C6667)?

There are two main differences between the two kits. Firstly, the CBQCA Protein Quantitation Kit (Cat. No. C6667) contains a potassium cyanide (KCN) component (component C). This component has been removed in the CBQCA Plus Protein Quantitation Kit (Cat. No. A66522) and replaced with mandelonitrile which serves as a less toxic alternative to KCN without compromising assay performance. Secondly, the CBQCA Plus Protein Quantitation Kit includes one additional component compared to the CBQCA Protein Quantitation Kit, a BupH Borate Buffer Pack.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

What is the shelf life of the BupH Borate Buffer Pack after reconstitution?

When properly reconstituted using a clean water source and good handling technique, the BupH Borate Buffer is stable for approximately 3-6 months when stored at 4 degrees C. For longer-term stability, we recommend adding sodium azide to a final concentration of 0.05% (w/v).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

What is the working range for the CBQCA Plus Protein Quantitation Kit?

The working range for the CBQCA assay is 50 ng - 150 µg protein.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

The CBQCA Plus Protein Quantitation Kit was shipped on wet ice but the storage recommendation is at -20 degrees C? Is it still good to use?

Yes, the product is still good to use. We ship many of our products at a higher temperature than the storage recommendation. The product will be stable for at least one week at the shipping temperature when in the original, unopened packaging. For long-term stability, the product should be placed at the recommended storage temperature upon receipt.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

Can I use the CBQCA Plus Protein Quantitation Kit to quantitate small peptides?

We do not recommend using the CBQCA assay for quantitating small peptides since the BSA standard used is not reflective of a peptide sample. A better assay to use would be the Pierce Quantitative Fluorometric Peptide Assay (Cat. No. 23290) or the Perce Quantitative Colorimetric Peptide Assay (Cat. No. 23275).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

Where can I find the expiration date for the CBQCA Plus Protein Quantitation Kit?

The CBQCA Plus Protein Quantitation Kit does not have an expiration date. The kit is guaranteed for 12 months from date of shipment, when stored as indicated in the user manual. Once the CBQCA is reconstituted in DMSO, the working reagent has a shelf life of 6 months.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

Should the CBQCA Plus Protein Quantitation Kit be read from the top or bottom on a plate reader?

The CBQCA assay is a suspension assay. Suspension assays should always be read with a top read on a plate reader for best sensitivity. Bottom reads will give reduced sensitivity.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

How do I know if the contaminants in my samples are compatible with the CBQCA Plus Protein Quantitation Kit?

For substances that do not contain primary amines and that we have not validated to be compatible with the CBQCA assay, we recommend preparing two standard curves, one with the substance spiked in at the desired concentration in the assay and one without the substance spiked in. If the substance does not significantly affect the quantitation of the assay, then you can use it in the assay. You should prepare the standard curve with the substance spiked in at the same concentration as used with the samples, so that the standards are under the same conditions.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

What is the compatibility of the CBQCA Plus Protein Quantitation Kit with urea or guanidine?

Both urea and guanidine contain primary amines, so neither are compatible with the CBQCA assay.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

Can the CBQCA Plus Protein Quantitation Kit be used on the Qubit Fluorometer?

The excitation/emission (Ex/Em) wavelengths of CBQCA are 465 nm and 550 nm respectively, which would fit within the blue excitation range of 430-495 nm and green emission range of 510-580 on the Qubit instruments, so it should be possible to set up a MyQubit assay for this reagent. Here is a link to the MyQubit guide.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.