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Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)
Invitrogen Lyo-ready RPA reagents are designed for fast, sensitive, and specific DNA amplification through isothermal recombinase polymerase amplification (RPA) reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats.
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| Catalog Number | Product Name | Quantity | Product Type |
|---|---|---|---|
| A72127 | Lyo-ready RPA Kit | 100 Reactions | DNA Amplification Kit |
| A72123 | Lyo-ready T4 Gene 32 Protein | 1600 μg | Stand-alone enzyme |
| A72124 | Lyo-ready T4 UvsX Protein | 120 μg | Stand-alone enzyme |
| A72125 | Lyo-ready T4 UvsY Protein | 120 μg | Stand-alone enzyme |
| A72126 | RPA Reaction Buffer (2X) | 2000 μL | Reaction Buffer |
| A72128 | Lyo-ready RPA Kit | 500 Reactions | DNA Amplification Kit |
Catalog number A72127
Price (USD)/ Each
263.00
-
Product Name:
Lyo-ready RPA Kit
Quantity:
100 Reactions
Product Type:
DNA Amplification Kit
Price (USD)/ Each
263.00
Lyo-ready Enzymes and Reagents for Recombinase Polymerase Amplification (RPA)
Catalog numberA72127
Price (USD)/ Each
263.00
-
Invitrogen Lyo-ready recombinase polymerase amplification (RPA) reagents are designed for fast, sensitive, and specific DNA amplification through isothermal RPA reactions. Lyo-ready RPA reagents are available in stand-alone and kit formats. Reverse transcriptase can be added to the RPA reaction for amplification of RNA targets. All reagents are glycerol-free, which enables assay lyophilization.
Features of Lyo-ready RPA products
- Enhanced sensitivity down to 1 copy using optimized (RT-)RPA reaction buffer
- Robust and specific RNA/DNA amplification
- Amplification from inhibitor-containing samples
- Fast target amplification in as little as 20 minutes
- Ability to easily optimize RPA or RT-RPA reactions
Kit components
The Lyo-ready RPA Kit includes all required components for an RPA reaction:
- Lyo-ready T4 UvsX Protein—produced from E. coli cells bearing cloned uvsX gene derived from bacteriophage T4
- Lyo-ready T4 UvsY Protein—a recombination mediator protein that stimulates homologous recombination performed by the UvsX recombinase
- Lyo-ready Bst DNA Polymerase (Cat. No. A56655)—a proprietary mutant of Bst DNA Polymerase, Large Fragment, with fast reaction speed, increased sensitivity, and tolerance to inhibitors
- Lyo-ready T4 Gene 32 Protein—a single-stranded DNA binding protein produced from E. coli cells carrying cloned bacteriophage T4 gene 32
- Optimized reaction buffer, magnesium chloride, and dNTPs
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeDNA Amplification Kit
FormLiquid
For Use With (Application)RPA, RT-RPA
PolymeraseLyo-ready Bst DNA Polymerase
Quantity100 Reactions
Research CategoryNucleic Acid Amplification
Optimal Reaction Temperature42°C
Product NameLyo-ready RPA Kit
Reverse TranscriptaseSuperScript IV Reverse Transcriptase
Shipping ConditionDry Ice
Detection MethodEnd point, real-time, lateral flow
Reaction TimeUp to 20 min.
Unit SizeEach
Contents & Storage
• Lyo-ready T4 UvsX Protein (1.2 mg/mL), 50 μL
• Lyo-ready T4 UvsY Protein (1.2 mg/mL), 50 μL
• Lyo-ready Bst DNA Polymerase (6 U/μL), 50 μL
• Lyo-ready T4 Gene 32 Protein (16 mg/mL), 50 μL
• 2X RPA Reaction Buffer, 1000 μL
• 280 mM MgCl2, 100 μL
• 10 mM dNTP Mix, 50 μL
Store at -25°C to -15°C.