Human (MDR1) and mouse (mdr1, mdr3) P-glycoproteins can be distinguished by their respective drug resistance profiles and sensitivity to modulators.
AuthorsTang-Wai DF, Kajiji S, DiCapua F, de Graaf D, Roninson IB, Gros P
JournalBiochemistry
PubMed ID7819214
Possible functional differences between P-glycoproteins (P-gps) encoded by the human MDR1 and mouse mdr1 and mdr3 genes with respect to drug resistance profiles and sensitivity to known modulators have been investigated. For this, the three genes were introduced and overexpressed in the same cellular background, that of Chinese hamster LR73 ... More
Transmission of cell stress from endoplasmic reticulum to mitochondria: enhanced expression of Lon protease.
AuthorsHori O, Ichinoda F, Tamatani T, Yamaguchi A, Sato N, Ozawa K, Kitao Y, Miyazaki M, Harding HP, Ron D, Tohyama M, M Stern D, Ogawa S
JournalJ Cell Biol
PubMed ID12082077
'The rat homologue of a mitochondrial ATP-dependent protease Lon was cloned from cultured astrocytes exposed to hypoxia. Expression of Lon was enhanced in vitro by hypoxia or ER stress, and in vivo by brain ischemia. These observations suggested that changes in nuclear gene expression (Lon) triggered by ER stress had ... More
Analysis of P-glycoprotein-mediated membrane transport in human peripheral blood lymphocytes using the UIC2 shift assay.
AuthorsPark SW, Lomri N, Simeoni LA, Fruehauf JP, Mechetner E
JournalCytometry A
PubMed ID12766968
'BACKGROUND: During transport-associated adenosine triphosphate hydrolysis, P-glycoprotein (Pgp) undergoes conformation transitions detected by UIC2, a functional anti-Pgp monoclonal antibody. A newly developed UIC2 shift assay is based on increased UIC2 reactivity in the presence of Pgp substrates. All peripheral blood leukocytes express low Pgp levels. The existing antibody-based detection methods ... More
Left-handed Z-DNA in bands of acid-fixed polytene chromosomes.
AuthorsArndt-Jovin DJ, Robert-Nicoud M, Zarling DA, Greider C, Weimer E, Jovin TM
JournalProc Natl Acad Sci U S A
PubMed ID6410390
'Antibodies to DNA in the left-handed (Z) conformation bind to acid-fixed polytene chromosomes of both Chironomus thummi and Drosophila melanogaster, as shown by direct and indirect immunofluorescence. Comparison of the phase-contrast, immunofluorescence, and DNA staining patterns shows a predominant localization of the antibody to the regions of high contrast and ... More
Neurotrophic factors prevent ceramide-induced apoptosis downstream of c-Jun N-terminal kinase activation in PC12 cells.
AuthorsHartfield PJ, Bilney AJ, Murray AW
JournalJ Neurochem
PubMed ID9648862
'Neurotrophic factors prevent apoptosis of PC12 cells in serum-free medium. The present study determines whether neurotrophic factors can prevent ceramide-induced apoptosis in PC12 cells and investigates the role that c-Jun N-terminal kinase (JNK) activation may play in this system. Ceramide-induced apoptosis was inhibited by nerve growth factor, basic fibroblast growth ... More
Amyloid beta-peptide induces cell monolayer albumin permeability, impairs glucose transport, and induces apoptosis in vascular endothelial cells.
AuthorsBlanc EM, Toborek M, Mark RJ, Hennig B, Mattson MP
JournalJ Neurochem
PubMed ID9109512
'Amyloid beta-peptide (A beta) is deposited as insoluble fibrils in the brain parenchyma and cerebral blood vessels in Alzheimer''s disease (AD). In addition to neuronal degeneration, cerebral vascular alterations indicative of damage to vascular endothelial cells and disruption of the blood-brain barrier occur in AD. Here we report that A ... More
A 116,000 Mr nucleolar antigen specific for the dense fibrillar component of the nucleoli.
AuthorsMasson C, Andre C, Arnoult J, Geraud G, Hernandez-Verdun D
JournalJ Cell Sci
PubMed ID2200792
'In ATT, a human autoimmune serum, we found anti-nucleolar antibodies that recognized nucleolar antigens confined to a single nucleolar compartment, the dense fibrillar component (DFC). We localized these antigens by immunoelectron microscopy in DFC of HeLa cell nucleoli both on Lowicryl sections and cryoultrathin sections without embedding. The antigens were ... More
DNA damage induced by tumour necrosis factor-alpha in L929 cells is mediated by mitochondrial oxygen radical formation.
AuthorsShoji Y, Uedono Y, Ishikura H, Takeyama N, Tanaka T
JournalImmunology
PubMed ID7790027
'Treatment of L929 cells with tumour necrosis factor-alpha (TNF-alpha) plus actinomycin D induced DNA damage (indicated by the appearance of a sub-G1 peak due to extracellular leakage of low molecular weight DNA following DNA fragmentation) before significant cell lysis occurred. The DNA damage occurred in parallel with a decrease of ... More
Chromatin interaction mechanism of transcriptional control in vivo.
AuthorsGribnau J, de Boer E, Trimborn T, Wijgerde M, Milot E, Grosveld F, Fraser P
JournalEMBO J
PubMed ID9774345
'We have used a kinetic analysis to distinguish possible mechanisms of activation of transcription of the different genes in the human beta globin locus. Based on in situ studies at the single-cell level we have previously suggested a dynamic mechanism of single genes alternately interacting with the locus control region ... More
Dissociation kinetics of actinomycin D from oligonucleotides with hairpin motifs.
AuthorsChen FM, Jones CM, Johnson QL
JournalBiochemistry
PubMed ID8504076
'The dissociation of 7-aminoactinomycin D (7-AM-ACTD) from d(ATGCATATGCAT), d(ATGCAT-T-ATGCAT), or d(ATGCAT-A-ATGCAT) at 20 degrees C cannot be adequately described by a single-exponential decay and requires a fit with two rate constants. The relative contributions of these two rate processes and their temperature dependence can be attributed to the coexistence of ... More
Control of neuronal size homeostasis by trophic factor-mediated coupling of protein degradation to protein synthesis.
AuthorsFranklin JL, Johnson EM
JournalJ Cell Biol
PubMed ID9732291
'We demonstrate that NGF couples the rate of degradation of long-lived proteins in sympathetic neurons to the rate of protein synthesis. Inhibiting protein synthesis rate by a specific percentage caused an almost equivalent percentage reduction in the degradation rate of long-lived proteins, indicating nearly 1:1 coupling between the two processes. ... More
Zebrafish vasa RNA but not its protein is a component of the germ plasm and segregates asymmetrically before germline specification.
AuthorsKnaut H, Pelegri F, Bohmann K, Schwarz H, Nüsslein-Volhard C
JournalJ Cell Biol
PubMed ID10811828
'Work in different organisms revealed that the vasa gene product is essential for germline specification. Here, we describe the asymmetric segregation of zebrafish vasa RNA, which distinguishes germ cell precursors from somatic cells in cleavage stage embryos. At the late blastula (sphere) stage, vasa mRNA segregation changes from asymmetric to ... More
Simultaneous flow cytometric measurement of viability and lymphocyte subset proliferation.
'Combined analysis of DNA content and immunofluorescence on single cells by flow cytometry provides information on the proliferative response of cellular sub-populations in mixed cell preparations. However, the presence of considerable numbers of dead (nonviable) cells impairs accurate flow cytometric data analysis, mainly, because dead cells can bind antibodies non-specifically ... More
The use of base pair specific DNA binding agents as affinity labels for the study of mammalian chromosomes.
AuthorsJorgenson KF, van de Sande JH, Lin CC
JournalChromosoma
PubMed ID81737
'The fluorochromes Hoechst 33258 and olivomycin are base pair specific DNA binding agents. The fluorescence enhancement of Hoechst 33258 and olivomycin in the presence of DNA can be directly related to the A--T and G--C content of the interacting DNA respectively. Cytological observations of metaphase chromosomes treated with these two ... More
Human monocytes induce a carcinoma cell line to secrete high amounts of nitric oxide.
AuthorsKonur A, Krause SW, Rehli M, Kreutz M, Andreesen R
JournalJ Immunol
PubMed ID8757334
'Nitric oxide (NO) is a short-lived pleiotropic mediator with a multitude of biologic functions. The inducible form of NO synthase (iNOS) is responsible for the discontinuous production of high amounts of NO and is important for the cytotoxic capacity of macrophages in rodents, whereas NO production by human macrophages or ... More
Calcium-activated DNA fragmentation kills immature thymocytes.
AuthorsMcConkey DJ, Hartzell P, Nicotera P, Orrenius S
JournalFASEB J
PubMed ID2497041
'Glucocorticoid hormones kill immature thymocytes by activating a self-destructive process that involves extensive DNA fragmentation. It has been demonstrated that thymocyte suicide is dependent on an early, sustained increase in cytosolic Ca2+ concentration, and new protein synthesis, but the biochemical lesion that leads to cell death has not been established. ... More
Selective binding of actinomycin D induces a reversible conformational transition of nucleosomes.
AuthorsCacchione S, Caneva R, Savino M
JournalBiochim Biophys Acta
PubMed ID3741874
'Equilibrium and hydrodynamic studies on the complex of actinomycin D with H1-H5 depleted, 175 basepair nucleosomes are reported. By spectral titration the intrinsic affinities of actinomycin D for nucleosomes and for DNA are found strictly comparable. Sedimentation analysis shows that actinomycin can apparently unfold the nucleosome, like ethidium bromide and ... More
Endogenous activation of apoptosis in bursal lymphocytes: inhibition by phorbol esters and protein synthesis inhibitors.
AuthorsCompton MM, Waldrip HM
JournalCell Immunol
PubMed ID9630840
'The bursa of Fabricius represents the primary immune organ where immature B cells undergo maturational changes in avian species. Isolation of bursal lymphocytes for analysis in cell culture results in the rapid endogenous activation of apoptosis. After 2 h of incubation, over 45% of the lymphocytes were shown to be ... More
7-Azido-actinomycin D: a photoaffinity probe of the sequence specificity of DNA binding by actinomycin D.
AuthorsRill RL, Marsch GA, Graves DE
JournalJ Biomol Struct Dyn
PubMed ID2627300
'Actinomycin D (ActD) is a DNA-binding antitumor antibiotic that appears to act in vivo by inhibiting RNA polymerase. The mechanism of DNA binding of ActD has attracted much attention because of its strong preference for 5''-dGpdC-3'' sequences. Binding is thought to involve intercalation of the tricyclic aromatic phenoxazone ring into ... More
Influence of DNA base sequence on the binding energetics of actinomycin D.
AuthorsBailey SA, Graves DE, Rill R, Marsch G
JournalBiochemistry
PubMed ID8504108
'The influences of base sequence on the thermodynamic properties associated with the interaction of actinomycin D with DNA are examined. It has been previously established that GpC steps of double-helical DNAs are highly preferred binding sites for actinomycin D. In this study, a series of oligonucleotides was designed and synthesized ... More
Flow cytometric competitive binding assay for determination of actinomycin-D concentrations.
AuthorsSaunders GC, Martin JC, Jett JH, Perkins A
JournalCytometry
PubMed ID2138531
'A single step, separation free competitive binding reaction between the fluorescent antibiotic mithramycin and actinomycin-D for common binding sites on DNA coated 10 microns diameter microspheres is described. The fluorescence of the microspheres is measured with a flowcytometer. In the presence of a constant amount of mithramycin, the microsphere fluorescence ... More
DNA recognition by intercalator-minor-groove binder hybrid molecules.
AuthorsBailly C, Hénichart JP
JournalBioconjug Chem
PubMed ID1666838
Actinomycin D binding to isolated deoxyribonucleoprotein and intact cells.
AuthorsBolund L
JournalExp Cell Res
PubMed ID4993099
Actinomycin binding capacity of deoxyribonucleoprotein.
AuthorsRingertz NR, Bolund L
JournalBiochim Biophys Acta
PubMed ID4885689
Isolation of selectively amplified DNA sequences from multidrug-resistant SEWA cells.
AuthorsStähl F, Sandberg P, Martinsson T, Skoog J, Dahllöf B, Wettergren Y, Bjursell G, Levan G
JournalHereditas
PubMed ID3583784
Studies of the binding of actinomycin and related compounds to DNA.
AuthorsMüller W, Crothers DM
JournalJ Mol Biol
PubMed ID4107107
Quantitating apoptosis by a nonradioactive DNA dot blot assay.
AuthorsHueber AO, Pierres M, He HT
JournalAnal Biochem
PubMed ID7810894
Fluorescence-based microplate bioassay for tumor necrosis factor.
AuthorsImrich A, Taylor M, Kobzik L
JournalJ Immunol Methods
PubMed ID9671159
Actinomycin D-binding in vivo: active chromatin preferred.
AuthorsYu FL
JournalFEBS Lett
PubMed ID6343119
Escherichia coli RNA polymerase and the endogenous engaged RNA polymerase I were used as specific probes to monitor the physiologically inactive and active nucleolar chromatin template function, respectively. Actinomycin D bound preferentially to the physiologically active regions of rat liver nucleolar chromatin in vivo. ... More
Chromatin and histones: binding of tritiated actinomycin D to heterochromatin in mealy bugs.
AuthorsBerlowitz L, Pallotta D, Sibley CH
JournalScience
PubMed ID5815043
The degree of actinomycin D binding to DNA in chromatin is dependent upon the state of repression of chromatin. Living cells bind three times more tritiated actinomycin to euchromatin than to genetically inactive heterochromatin. Extraction of histone results in a general increase in tritiated actinomycin binding and in a ratio ... More
Phase II study of bleomycin, actinomycin D, DTIC and vindesine in disseminated malignant melanoma.
Twenty-seven patients with disseminated malignant melanoma were treated with combination chemotherapy consisting of bleomycin, actinomycin D, DTIC and vindesine. There were 4 complete responses in patients with pulmonary metastasis, 5 patients had a partial remission, 2 of them had mainly lung lesions. Toxicity consisted mainly of leuco- and thrombocytopenia of ... More
Reversible depression of spontaneous brain electrical activity induced by actinomycin D and 7-aminoactinomycin D in rats.
AuthorsUezu E, Takada Y, Mishima N
JournalExperientia
PubMed ID7151959
Various types of actinomycin (C,D,S2, I and V) and 7-amino-analogue of actinomycin D were injected into the right lateral ventricle of the brain through a chronically implanted cannula. In rats but not in mice actinomycin D, actinomycin S2 and 7-aminoactinomycin D caused depression of EEG, while cardiac and respiratory activity ... More
Pharmaceutical-induced cell apoptosis characterized by capillary zone electrophoresis.
AuthorsLiu BF, Zhang L, Liu HH, Lu YT
JournalAnal Biochem
PubMed ID11567522
Capillary zone electrophoresis (CZE) with a non-gel-sieving system was employed to characterize actinomycin D-induced cell apoptosis by measuring cellular DNA damage, termed DNA ladder, which proved to be thoroughly different from the DNA damage pattern of cell necrosis. The results by CZE analyses were identical to those obtained by conventional ... More
Binding of actinomycin D to DNA: evidence for a nonclassical high-affinity binding mode that does not require GpC sites.
We have employed a combination of temperature-dependent UV absorption spectroscopy, circular dichroism, and batch calorimetry to characterize the binding of actinomycin D to a series of oligomeric DNA duplexes. We find the duplex [d(CGTCGACG)]2 to be unique in its ability to bind actinomycin D strongly despite the absence of a ... More
Synaptic regulation of glial protein expression in vivo.
We investigated signaling between individual nerve terminals and perisynaptic Schwann cells, the teloglial cells that cover neuromuscular junctions. When deprived of neuronal activity in vivo, either by motor nerve transection or tetrodotoxin injection, perisynaptic Schwann cells rapidly up-regulated glial fibrillary acidic protein. Addition of transcription or translation inhibitors to excised ... More
Macromolecular synthesis inhibitors prevent oxidative stress-induced apoptosis in embryonic cortical neurons by shunting cysteine from protein synthesis to glutathione.
AuthorsRatan RR, Murphy TH, Baraban JM
JournalJ Neurosci
PubMed ID8027786
Although macromolecular synthesis inhibitors have been demonstrated to prevent neuronal apoptosis in a number of paradigms, their mechanisms of protection remains unclear. Recently, we found that neuronal death resulting from cystine deprivation, glutathione loss, and oxidative stress is apoptotic and is prevented by inhibitors of macromolecular synthesis. We now report ... More
Monitoring of relative mitochondrial membrane potential in living cells by fluorescence microscopy.
AuthorsJohnson LV, Walsh ML, Bockus BJ, Chen LB
JournalJ Cell Biol
PubMed ID6783667
Permeant cationic fluorescent probes are shown to be selectively accumulated by the mitochondria of living cells. Mitochondria-specific interaction of such molecules is apparently dependent on the high trans-membrane potential (inside negative) maintained by functional mitochondria. Dissipation of the mitochondrial trans-membrane and potential by ionophores or inhibitors of electron transport eliminates ... More
A cytotoxicity assay for tumor necrosis factor employing a multiwell fluorescence scanner.
AuthorsTrost LC, Lemasters JJ
JournalAnal Biochem
PubMed ID7978238
Tumor necrosis factor (TNF) is an important mediator of many biological processes. This study sought to develop a sensitive bioassay allowing quantitative determination of TNF. The assay is based on uptake of the membrane impermeant dye, propidium iodide, by L929 cells when their viability is lost. L929 cells were plated ... More
7-Azidoactinomycin D: a novel probe for examining actinomycin D-DNA interactions.
AuthorsGraves DE, Wadkins RM
JournalJ Biol Chem
PubMed ID2708366
The technique of photoaffinity labeling is applied to the actinomycin D system to provide a novel probe for the examination of the interactions of actinomycin D with nucleic acids. The capacity for covalent attachment of actinomycin D will aid greatly in the study of target-site specificities and the correlations of ... More
N-acetylcysteine-promoted survival of PC12 cells is glutathione-independent but transcription-dependent.
AuthorsYan CY, Ferrari G, Greene LA
JournalJ Biol Chem
PubMed ID7592924
Our prior work established that comparable concentrations of N-acetylcysteine (NAC) both block the proliferation of PC12 cells and prevent death of trophic factor-deprived sympathetic neurons and PC12 cells. The present work addresses several aspects of the mechanisms of these actions. NAC increases intracellular levels of glutathione (GSH) by approximately 10-fold ... More
Distinct functions of nuclear and cytoplasmic calcium in the control of gene expression.
AuthorsHardingham GE, Chawla S, Johnson CM, Bading H
JournalNature
PubMed ID9000075
Calcium entry into neuronal cells through voltage or ligand-gated ion channels triggers neuronal activity-dependent gene expression critical for adaptive changes in the nervous system. Cytoplasmic calcium transients are often accompanied by an increase in the concentration of nuclear calcium, but the functional significance of such spatially distinct calcium signals is ... More
Oxidized low-density lipoprotein decreases the expression of endothelial nitric oxide synthase.
AuthorsLiao JK, Shin WS, Lee WY, Clark SL
JournalJ Biol Chem
PubMed ID7529227
The atherogenic effects of low-density lipoprotein (LDL) may be mediated, in part, by its effect(s) on endothelial-derived nitric oxide (NO). To determine whether LDL can modulate NO production by changing NO synthase expression, we treated human saphenous vein endothelial cells with increasing concentrations of native or oxidized LDL (0-100 micrograms/ml) ... More
Enzyme-linked immunosorbent assay for the quantification of actinomycin D using beta-D-galactosidase as a label.
AuthorsFujiwara K, Saita T, Takenawa N, Matsumoto N, Kitagawa T
JournalCancer Res
PubMed ID3136914
An enzyme-linked immunosorbent assay (ELISA) for actinomycin D (AMD) has been developed, which allowed us to measure accurately as little as 50 pg of AMD per assay well. Anti-AMD sera was obtained by immunizing rabbits with an AMD derivative, 7-aminoactinomycin D (7AMD), conjugated with mercaptosuccinyl bovine serum albumin via N-maleoylaminobutyric ... More
Efficient mammalian protein synthesis requires an intact F-actin system.
AuthorsStapulionis R, Kolli S, Deutscher MP
JournalJ Biol Chem
PubMed ID9312103
The mammalian protein synthesizing system is highly organized in vivo, and its substrate, tRNA, is channeled throughout the translation process. However, the cellular components responsible for this organization are not known. To examine this question a series of studies was carried out using intact and permeabilized Chinese hamster ovary cells. ... More
Distinct regions in the 3' untranslated region are responsible for targeting and stabilizing utrophin transcripts in skeletal muscle cells.
AuthorsGramolini AO, Bélanger G, Jasmin BJ
JournalJ Cell Biol
PubMed ID11551978
In this study, we have sought to determine whether utrophin transcripts are targeted to a distinct subcellular compartment in skeletal muscle cells, and have examined the role of the 3' untranslated region (UTR) in regulating the stability and localization of utrophin transcripts. Our results show that utrophin transcripts associate preferentially ... More
Crystal structure of the 2:1 complex between d(GAAGCTTC) and the anticancer drug actinomycin D.
AuthorsKamitori S, Takusagawa F
JournalJ Mol Biol
PubMed ID1593629
The crystal structures of the 2:1 complex of the self-complementary DNA octamer d(GAAGCTTC) with actinomycin D has been determined at 3.0 A resolution. This is the first example of a crystal structure of a DNA-drug complex in which the drug intercalates into the middle of a relatively long DNA segment. ... More
A flow-cytometric method for the separation and quantitation of normal and apoptotic thymocytes.
Using flow cytometry, we describe a method for separating and quantifying normal and apoptotic thymocytes. Apoptosis was induced in isolated thymocytes from immature rats by treatment with the glucocorticoid dexamethasone or the antitumor agent etoposide. Subsequent incubation with the vital bisbenzimidazole dye Hoechst 33342 and the DNA intercalating agent propidium ... More
Circumvention of P-glycoprotein-mediated multiple drug resistance by phosphorylation modulators is independent of protein kinases.
AuthorsSmith CD, Zilfou JT
JournalJ Biol Chem
PubMed ID7499304
Expression of P-glycoprotein by tumor cells confers resistance to multiple natural product drugs because of its ability to export these compounds. This transporter is a substrate for several protein kinases; however, the functional significance of its phosphorylation is not defined. We examined the effects of many activators and inhibitors of ... More
S100A13 participates in the release of fibroblast growth factor 1 in response to heat shock in vitro.
AuthorsLandriscina M, Soldi R, Bagalá C, Micucci I, Bellum S, Tarantini F, Prudovsky I, Maciag T
JournalJ Biol Chem
PubMed ID11410600
S100A13, a member of the S100 gene family of Ca(2+)-binding proteins has been previously characterized as a component of a brain-derived heparin-binding multiprotein aggregate/complex containing fibroblast growth factor 1 (FGF1). We report that while expression of S100A13 in NIH 3T3 cells results in the constitutive release of S100A13 into the ... More
Sequence-specific actinomycin D binding to single-stranded DNA inhibits HIV reverse transcriptase and other polymerases.
AuthorsRill RL, Hecker KH
JournalBiochemistry
PubMed ID8639503
Primer extension assays using recombinant templates constructed to contain all 256 possible base quartets in a minimum length sequence were used to examine binding of the anticancer drug actinomycin D to single-stranded DNA. Single-stranded templates were generated by digestion of linearized plasmid with the double-strand-specific T7 gene 6 exonuclease. Actinomycin ... More
Counterstain-enhanced chromosome banding.
AuthorsSchweizer D
JournalHum Genet
PubMed ID6167505
Chromosome staining, in which at least one member of a pair or triplet of DNA binding dyes is fluorescent whereas the others act as counterstain, is reviewed. Appropriately chosen combinations of fluorescent dyes and counterstains can be employed to enhance general chromosome banding patterns, or to induce specific regional banding ... More
Actinomycin D binds to metastable hairpins in single-stranded DNA.
AuthorsWadkins RM, Vladu B, Tung CS
JournalBiochemistry
PubMed ID9718315
We have examined the role of DNA composition in the binding of actinomycin D to single-stranded DNA. By using the fluorescent analogue 7-aminoactinomycin D, we were able to monitor binding of the drug to ssDNA with single base changes distant from the 5'-TAGT-3' site previously determined to be a high-affinity ... More
Use of fluorescent dyes as molecular probes for the study of multidrug resistance.
AuthorsNeyfakh AA
JournalExp Cell Res
PubMed ID3335222
Fluorescence microscopy has shown that 18 different fluorescent dyes, staining various intracellular structures in transformed hamster fibroblasts (DM-15), did not stain or stained weakly multidrug-resistant cells selected from DM-15 by colchicine. Reduced staining by fluorescent dyes was characteristic also of five other tested multidrug-resistant cell lines of hamster and mouse ... More
IFN-alpha beta reconstitutes the deficiency in lipid A-activated AKR macrophages for nitric oxide synthase.
AuthorsJiang H, Rummage JA, Zhou A, Chen Z, Herriot MJ, Stewart CA, Kolosov M, Leu RW
JournalJ Immunol
PubMed ID8683131
AKR mouse peritoneal macrophages (PM) were previously found to have a defect in their response to lipid A for nitric oxide (NO)-mediated tumor cytotoxicity, which was related to a lower level of C1q synthesis and reconstituted by exogenous IFN-gamma or C1q. We used AKR-PM as a model to further define ... More
Targeting of U2AF65 to sites of active splicing in the nucleus.
AuthorsGama-Carvalho M, Krauss RD, Chiang L, Valcárcel J, Green MR, Carmo-Fonseca M
JournalJ Cell Biol
PubMed ID9166400
U2AF65 is an essential splicing factor that promotes binding of U2 small nuclear (sn)RNP at the pre-mRNA branchpoint. Here we describe a novel monoclonal antibody that reacts specifically with U2AF65. Using this antibody, we show that U2AF65 is diffusely distributed in the nucleoplasm with additional concentration in nuclear speckles, which ... More
Cyanide-induced apoptosis and oxidative stress in differentiated PC12 cells.
AuthorsMills EM, Gunasekar PG, Pavlakovic G, Isom GE
JournalJ Neurochem
PubMed ID8752110
Terminally differentiated PC12 cells are a useful neuron-like model for studying programmed cell death in response to nerve growth factor (NGF) deprivation. This in vitro model was used to investigate the mechanism by which cyanide-induced histotoxic hypoxia produces neuronal degeneration. Treatment of undifferentiated PC12 cells with 0.1 mM KCN for ... More
Peripheral V gamma 9/V delta 2 T cell deletion and anergy to nonpeptidic mycobacterial antigens in asymptomatic HIV-1-infected persons.
AuthorsPoccia F, Boullier S, Lecoeur H, Cochet M, Poquet Y, Colizzi V, Fournie JJ, Gougeon ML
JournalJ Immunol
PubMed ID8683151
Gamma delta T cells represent a minor population of human peripheral lymphocytes, the majority of them expressing the V delta 2/V gamma 9 TCR. Their accumulation in infectious disease lesions and their reactivity toward mycobacterial Ags suggest that V gamma 9/V delta 2 T cells play a role during infectious ... More
Glia modulate the response of murine cortical neurons to excitotoxicity: glia exacerbate AMPA neurotoxicity.
AuthorsDugan LL, Bruno VM, Amagasu SM, Giffard RG
JournalJ Neurosci
PubMed ID7540679
We have developed "pure" neuronal cultures (< 1% astrocytes) from mouse neocortex to study the effect of glial cells on the response of neurons to injury. Cortical neurons were found to require glial-conditioned medium to survive. Immature neurons, 2-4 d in vitro, deprived of glial-conditioned medium, underwent apoptosis over 48 ... More
Effects of glucocorticoids on Na+/H+ exchange and growth in cultured vascular smooth muscle cells.
AuthorsBerk BC, Vallega G, Griendling KK, Gordon JB, Cragoe EJ, Canessa M, Alexander RW
JournalJ Cell Physiol
PubMed ID2461379
We have examined the effects of hydrocortisone on growth and Na+/H+ exchange in cultured rat aortic vascular smooth muscle cells (VSMC). Hydrocortisone (2 microM) treatment of growth-arrested VSMC significantly decreased VSMC growth in response to 10% calf serum assayed by 3H-thymidine incorporation and cell number at confluence. This effect was ... More
Binding of actinomycin D to the T(G)nT motif of double-stranded DNA: determination of the guanine requirement in nonclassical, non-GpC binding sites.
AuthorsBailey SA, Graves DE, Rill R
JournalBiochemistry
PubMed ID7918362
Strong binding of the antitumor antibiotic actinomycin D to the sequence 5'-TGGGT-3' in double-stranded DNA was recently established by equilibrium binding studies (Bailey et al., 1993). Actinomycin D binding to this -TGGGT- containing sequence was shown to be comparable to that of an -XGCY- containing oligonucleotide (Ka approximately 10(6) M-1). ... More
Apoptosis associated with ex vivo down-regulation of Bcl-2 and up-regulation of Fas in potential cytotoxic CD8+ T lymphocytes during HIV infection.
AuthorsBoudet F, Lecoeur H, Gougeon ML
JournalJ Immunol
PubMed ID8690919
In this study, we have investigated whether the enhanced apoptosis of CD4+ and CD8+ T lymphocytes throughout HIV infection was controlled by the bcl-2 proto-oncogene, an inhibitor of programmed cell death (PCD) in mammals. We have analyzed the intracellular expression of the Bcl-2 protein by flow cytometry in freshly isolated ... More
Enhancement of Mdr2-mediated phosphatidylcholine translocation by the bile salt taurocholate. Implications for hepatic bile formation.
AuthorsRuetz S, Gros P
JournalJ Biol Chem
PubMed ID7592705
Expression of the Mdr2-protein in secretory vesicules (SVs) from the yeast mutant sec6-4 causes a time- and temperature-dependent enhancement of phosphatidylcholine (PC) translocation from the outer to the inner leaflet of the SV lipid bilayer. We show that this activity is independent of changes either in the membrane potential or ... More
Membrane topology of a cysteine-less mutant of human P-glycoprotein.
AuthorsLoo TW, Clarke DM
JournalJ Biol Chem
PubMed ID7822320
A human P-glycoprotein devoid of cysteine residues was constructed by site-directed mutagenesis for studying its topology. The cDNA for human P-glycoprotein-A52 in which codons for cysteines 137, 431, 717, 956, 1074, 1125, 1227, 1288, and 1304 were changed to Ala, was transfected into NIH 3T3 cells and analyzed with respect ... More
Neurotrophins rescue cerebellar granule neurons from oxidative stress-mediated apoptotic death: selective involvement of phosphatidylinositol 3-kinase and the mitogen-activated protein kinase pathway.
AuthorsSkaper SD, Floreani M, Negro A, Facci L, Giusti P
JournalJ Neurochem
PubMed ID9572269
Cerebellar granule neurons maintained in medium containing serum and 25 mM K+ reliably undergo an apoptotic death when switched to serum-free medium with 5 mM K+. New mRNA and protein synthesis and formation of reactive oxygen intermediates are required steps in K+ deprivation-induced apoptosis of these neurons. Here we show ... More
Chronic extracellular acidosis induces plasmalemmal vacuolar type H+ ATPase activity in osteoclasts.
Proton extrusion into an extracellular resorption compartment is an essential component of bone degradation by osteoclasts. Chronic metabolic acidosis is known to induce negative calcium balance and bone loss by stimulating osteoclastic bone resorption, but the underlying mechanism is not known. The present studies were undertaken to evaluate whether chronic ... More
The Interaction of the Tumor Suppressor FAM46C with p62 and FNDC3 Proteins Integrates Protein and Secretory Homeostasis.
Authors
JournalCell Rep
PubMed ID32966780
Piwil1 Regulates Glioma Stem Cell Maintenance and Glioblastoma Progression.
Authors
JournalCell Rep
PubMed ID33406417
Oligonucleotide capture sequencing of the SARS-CoV-2 genome and subgenomic fragments from COVID-19 individuals.
Authors
JournalbioRxiv
PubMed ID32766579
Oligonucleotide capture sequencing of the SARS-CoV-2 genome and subgenomic fragments from COVID-19 individuals.
Authors
JournalPLoS One
PubMed ID34432798
Insulin regulates retinol dehydrogenase expression and all-trans-retinoic acid biosynthesis through FoxO1.
Authors
JournalJ Biol Chem
PubMed ID25627686
AMPK-SIRT1-independent inhibition of ANGPTL3 gene expression is a potential lipid-lowering mechanism of metformin.
Authors
JournalJ Pharm Pharmacol
PubMed ID31273790
A Rapid Induction Mechanism for Lin28a in Trophic Responses.