Thermo-Start DNA Polymerase, with 10x High Performance buffer and separate vial of 25 mM MgCl2

Catalog number: AB1057B

Thermo Scientific™  Related applications: PCR

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Thermo Scientific Thermo-Start Taq DNA Polymerase is a chemically modified version of ThermoPrime Taq DNA Polymerase that remains completely inactive during reaction set up to prevent non-specific amplification and primer dimer formation. This enzyme requires an activation step at 95°C for 15 minutes.


• Hot start modification ensures stringent specificity
• Safe for room temperature reaction setup
• Enhanced enzyme stability for increased yield and sensitivity
• Advanced buffering system minimizes PCR optimization steps


• Amplifying fragments up to 5 kb
• Hot start PCR

Related Products
Thermo-Start DNA Polymerase, with 10x buffer and separate vial of 25 mM MgCl2
ThermoPrime 2x ReddyMix PCR Master Mix, with 1.5 mM MgCl2
For Research Use Only. Not for use in diagnostic procedures.


Polymerase: Thermo-Start DNA Polymerase
Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 1 X
GC-Rich PCR Performance: Low
Reaction Format: Standalone
Product Overhang: 3'-A
Reaction Speed: Standard
Product Size: 2500 units

Contents & storage

• 10 x 50 µL Thermo-Start Taq DNA Polymerase (5 U/µL)
• 10 x 1.5 mL 10X Thermo-Start High Performance PCR Buffer
• 10 x 1.5 mL 25 mM MgCl2

Store at -20°C.


Manuals & protocols