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      ABsolute QPCR Mix, SYBR Green, fluorescein

      Catalog number: AB1219B

      Thermo Scientific™  Related applications: Real Time PCR (qPCR)

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      Description

      **********************
      Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of ABsolute QPCR Mix, SYBR Green, and added additional capabilities for your gene expression analysis.

      **********************
      Thermo Scientific ABsolute qPCR SYBR Green Mixes are optimized for SYBR Green chemistry and contain all the components necessary to perform quantitative PCR, with the exception of template and primers. The 2X qPCR SYBR Green Mix contains optimal levels of active SYBR Green I dye and Thermo-Start DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets.

      ABsolute qPCR SYBR Green Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, Fluorescein or no reference dye. A master mix optimized for capillary instruments is also available.

      Highlights

      • High Sensitivity & Specificity—The master mixes contain our patented hot start enzyme Thermo-Start DNA Polymerase
      • Optimised buffer containing SYBR Green I
      • Reproducibility—All master mixes undergo extensive QC to ensure batch to batch consistency
      • Broad dynamic range

      Applications

      • Gene expression analysis
      • siRNA validation
      • Genotyping

      Related Products
      ABsolute QPCR Mix, no ROX
      ABsolute QPCR Mix, low ROX
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      ABsolute QPCR Capillary Mix
      ABsolute QPCR Mix, SYBR Green, no ROX
      ABsolute QPCR Mix, SYBR Green, low ROX
      ABsolute QPCR Mix, SYBR Green, ROX
      ABsolute QPCR Mix, SYBR Green, fluorescein
      ABsolute QPCR Capillary Mix, SYBR Green
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Detection Method: SYBR
      For Use With (Equipment): BioRad MyiQ, BioRad iCycler iQ, BioRad iQ5
      Reaction Speed: Standard
      PCR Method: qPCR
      Sample Type (General): DNA, dsDNA
      Number of Reactions: 1,600 Reactions
      Polymerase: Taq DNA Polymerase
      Product Line: ABsolute
      Product Size: 1,600 reactions

      Contents & storage

      Contains:
      • 16 x 1.25 mL ABsolute QPCR SYBR Green Fluorescein Mix (2X)
      • 100 µL MgCl2 (1M)
      Sufficient for 1600 x 25 µL reactions.

      Store at -20°C

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