What kits should I use to isolate RNA from my sample?
The best isolation kit to use will depend greatly on your application, but, in general, the kits below produce RNA input compatible with the Ion Total RNA-Seq Kit v2 workflow:
Whole Transcriptome (polyA-selected or rRNA-depleted RNA is highly recommended, but total RNA is acceptable)
- Poly A-selected RNA
• Dynabeads mRNA DIRECT Micro Kit (Cat. No. 61021)
• MicroPoly(A)Purist Kit (Cat. No. AM1919)
- rRNA-depleted RNA
• RiboMinus Eukaryote System v2 (Cat. No. A15026)
• Low Input RiboMinus Eukaryote System v2 (Cat. No. A15027)
- Total RNA
• TRI Reagent
• TRizol
• RecoverAll MagMax
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.
Whole Transcriptome (polyA-selected or rRNA-depleted RNA is highly recommended, but total RNA is acceptable)
- Poly A-selected RNA
• Dynabeads mRNA DIRECT Micro Kit (Cat. No. 61021)
• MicroPoly(A)Purist Kit (Cat. No. AM1919)
- rRNA-depleted RNA
• RiboMinus Eukaryote System v2 (Cat. No. A15026)
• Low Input RiboMinus Eukaryote System v2 (Cat. No. A15027)
- Total RNA
• TRI Reagent
• TRizol
• RecoverAll MagMax
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.