Kit de transcripción mMESSAGE mMACHINE™ T3

Obtiene entre 10–50 veces la producción de reacciones de transcripción in vitro convencionales

Número de catálogo	Tipo de producto	N.º de reacciones
AM1348	Kit de transcripción in vitro T3	25 reacciones

Número de catálogo AM1348

Precio (MXN)

Tipo de producto:

Kit de transcripción in vitro T3

N.º de reacciones:

25 reacciones

mMESSAGE mMACHINE high yield capped RNA transcription kits use patented high-yield transcription technology for the routine synthesis of 15–35 μg of 7-methyl guanosine capped RNA from 1 μg of template DNA. Each kit includes sufficient reagents for 25 reactions.

High yields of capped RNA—up to 35 μg of RNA per reaction
Extra convenience—cap analog-nucleotide mix provided
Simplified reaction format
Synthesized transcripts ideal for microinjection, in vitro translation, or transfection
10–50 times yield obtained with conventional in vitro transcription reactions
Ratio of cap analog to GTP has been optimized to allow compromise between yield (15–35 μg) and proportion of transcripts that are capped (80%)
Contains a LiCl precipitation solution, efficient for separating proteins and unincorporated nucleotides (including free cap analog) from the capped RNA, allowing an increased efficiency of translation
Optimized for use with polymerases included in kit
Contains 57 U of cap analog

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Para utilizar con (aplicación)Transcripción in vitro, ARNm sintético, ARNm, transfección, LNP, GENEArt, plasmídico de ADN linealizado, suministro, productos de PCR

N.º de reacciones25 reacciones

Línea de productosAmbion, mMESSAGE mMACHINE

Tipo de productoKit de transcripción in vitro T3

PromotorT3

Cantidad25 reacciones

Condiciones de envíoHielo seco

Material de partidaProductos de PCR, plásmido de ADN linealizado

Producción20-30 μg

Tipo de producto finalARNm sintético, ARNm

FormatoKit

Tipo de muestraARNm, ARNm sintético

Unit SizeEach

Contenido y almacenamiento

Mezcla de enzimas T3, tampón de reacción 10X 2XNTP⁄CAP, solución ATP, solución CAP, solución GTP, pTRI-Xef, ADNasa TURBO, solución de parada de acetato de amonio, solución de cloruro de litio y tampón de carga de gel II. Todos se almacenan a -20°C. El agua sin nucleasas puede almacenarse a cualquier temperatura.

Preguntas frecuentes

I am using the mMESSAGE mMACHINE T3 Transcription Kit for in vitro transcription. I ran my reaction product on a denaturing gel and got a smear. Can you offer some advice?

If the RNA appears degraded (e.g. smeared), remove residual RNase from the DNA template preparation before in vitro transcription. Do this by digesting the DNA prep with proteinase K (100-200 µg/mL) in the presence of 0.5% SDS for30 min at 50°C, follow this with phenol/chloroform extraction. The RNase Inhibitor that is present in the transcription reaction, can only inactivate trace RNase contamination. Large amounts of RNase contamination will compromise the size and amount of transcription products.