mMESSAGE mMACHINE® T3 Transcription Kit

Catalog number: AM1348

Ambion™  Related applications: In Vitro Transcription

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Description

Ambion® mMESSAGE mMACHINE® High Yield Capped RNA Transcription Kits use Ambion's patented high-yield transcription technology for the routine synthesis of 15–35 µg of 7-methyl guanosine capped RNA from 1 µg of template DNA. Each kit includes sufficient reagents for 25 reactions.
• High yields of capped RNA—up to 39 µg of RNA per reaction
• Extra convenience—cap analog-nucleotide mix provided
• Simplified reaction format
• Synthesized transcripts ideal for microinjection, in vitro translation, or transfection In a 20 µl reaction during a 2 hour incubation, mMESSAGE mMACHINE® High Yield Capped RNA Transcription will yield up to 35 µg of capped RNA. This is 10–50 times the yield obtained with conventional in vitro transcription reactions. The ratio of cap analog to GTP has been optimized to allow the best compromise between yield (15–35 µg) and proportion of transcripts that are capped (80%). The mMESSAGE mMACHINE® kits also contain a LiCl precipitation solution that is efficient for separating proteins and unincorporated nucleotides (including free cap analog) from the capped RNA, allowing an increased efficiency of translation. mMESSAGE mMACHINE® Kits are only optimized for use with the polymerases included in the kit. Using a different polymerase than what is supplied in the kit may result in low yields. Each mMESSAGE mMACHINE® Kit contains 57 U of cap analog, an outstanding value for the cost.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Starting Material: Linearized DNA, PCR Products
Promoter: T3
RNA Yield: 20-30 µg
Final Product: Capped RNA, In Vitro Transcribed RNA
Product Size: 25 reactions
Shipping Condition: Dry Ice

Contents & storage

T3 Enzyme Mix, 10X Reaction Buffer 2XNTP⁄CAP Solution, GTP Solution, pTRI-Xef, TURBO DNase, Ammonium Acetate Stop Solution, Lithium Chloride Precipitation Solution, and Gel Loading Buffer II are all stored at –20°C. Nuclease-free Water may be stored at any temperature.

Documents

Manuals & protocols