Silencer® siRNA Construction Kit with manual

Catalog number: AM1620M

Ambion™  Related applications: RNAi

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The Ambion® Silencer® siRNA Construction Kit is for the synthesis of siRNAs by in vitro transcription of DNA templates and costs less per siRNA than chemical synthesis. The kit includes sufficient reagents to synthesize 15 siRNAs.
• Cut siRNA preparation time—generate up to 15 siRNAs in less than 24 hours
• Get hundreds of transfections per reaction
For Research Use Only. Not for use in diagnostic procedures.


Control Type: Transcription Control
Product Size: 15 reactions
Sample Type (Specific): In Vitro Transcribed siRNA

Contents & storage

DNA Hybridization Buffer, T7 Promoter Primer, Klenow Reaction Buffer, 10X dNTP, Exo– Klenow, T7 Enzyme Mix, T7 Reaction Buffer, 2X NTP Mix, Digestion Buffer, DNase, RNase, Sense Control DNA, and Antisense Control DNA should be stored at –20°C. siRNA Binding Buffer, siRNA Wash Buffer, Filter Cartridges, and Tubes should be stored at room temperature. Nuclease-free Water may be stored at any temperature.


Manuals & protocols