Invitrogen™

Silencer™ siRNA Starter Kit

Catalog number: AM1640
Invitrogen™

Silencer™ siRNA Starter Kit

Catalog number: AM1640
Catalog Number
AM1640
Unit Size
1 kit
Price (USD)
612.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
AM16401 kit
612.00
Your Price:
***
Product Overview
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Perfect for researchers new to RNAi, this Ambion™ kit contains all of the reagents, protocols, and instructions necessary to get underway with siRNA transfection and ensure successful gene knockdown experiments.

• The perfect choice for those beginning siRNA experiments
• Includes transfection agent, siRNAs, and three different systems for assessing knockdown
• For use with human, mouse, and rat cells

The Silencer™ siRNA Starter Kit includes a high-quality, lipid-based transfection agent, siPORT™ NeoFX™, that effectively delivers siRNAs to a broad range of cell lines. It can be used for reverse transfection, a streamlined protocol that eliminates an entire day from the RNAi experimental procedure; it can also be used with standard transfection protocols. This reagent is not sensitive to serum and efficiently transfects low concentrations of siRNA. The kit also includes a well-characterized GAPDH siRNA, which has been shown to strongly down-regulate GADPH in human, mouse, and rat cells, as well as a nontargeting negative control siRNA, Silencer™ Negative Control #1 siRNA. Because GAPDH is ubiquitously expressed and is easy to detect at both the mRNA and protein level, it is an ideal target for a positive control siRNA.

Three Detection Systems
The kit includes three different systems for monitoring GAPDH siRNA-induced knockdown. An anti-GAPDH antibody is provided to assess GAPDH protein levels by western blot or immunofluorescence. The KDalert™ GAPDH Assay is also provided to quickly measure GAPDH enzymatic activity. This assay can also be used to assess cell viability. Finally, RT-PCR primers are included, for real-time quantitative RT-PCR detection of GAPDH mRNA using SYBR™ Green. These reagents can also be used to transfect miRNAs, such as the Ambion™ Pre-miR™ miRNA Precursor Molecules.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Control Type
Negative Control, Positive Control
For Use With (Application)
Transfection
Label or Dye
SYBR Green
Product Line
Silencer™, Ambion™
Product Type
siRNA Starter Kit
Quantity
1 Kit
RNAi Type
siRNA, miRNA
Format
Kit
Sample Type
Synthetic siRNA, miRNA
Transfection Technique
Lipid-Based Transfection

Contents & Storage

Anti-GAPDH Monoclonal Antibody, GAPDH PCR Primers, KDalert™ Lysis Buffer, KDalert™ Solution A, KDalert™ Solution B, and KDalert™ Solution C should be stored at –20°C. GAPDH enzyme, GAPDH siRNA, and Negative Control #1 siRNA should be stored at below –70°C. siPORT™ NeoFX™ Transfection Agent should be stored at 4°C. Nuclease-free water may be stored at any temperature.

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