Ambion™ Cy™3 dye-labeled synthetic miRNA mimic for miRNA functional analysis. 5 nmol is supplied.
• Optimized design results in correct strand utilization by RISC
• Purified and ready to transfect
Ambion™ Pre-miR™ miRNA Precursor Molecules are small, chemically modified, double-stranded RNA molecules designed to mimic endogenous mature miRNAs. This ready-to-use miRNA mimic can be introduced into cells using transfection or electroporation parameters similar to those used for siRNAs. They enable detailed study of miRNA biological effects via gain-of-function experiments. The Cy™3 Dye-Labeled Pre-miR™ Negative Control #1 is a random sequence Pre-miR™ molecule that has been extensively tested in human cell lines and tissues and validated to produce no identifiable effects on known miRNA function. It is ideal for monitoring transfection efficiency, as the fluorescent label enables direct observation of the cellular uptake, distribution, and localization of the control. The label is on the 5' end of the molecule, and the oligonucleotide sequence is the same as the Pre-miR™ miRNA Precursor Molecule Negative Control #1 (SKU #AM17110). NOTE: Pre-miR™ miRNA Precursors are not hairpin constructs and should not be confused with pre-miRNAs.