Invitrogen™

Cy3™ Dye-Labeled Pre-miR Negative Control #1

Katalognummer	Menge
AM17120	5 nmol

Katalognummer AM17120

Preis (EUR)

370,00

Menge:

5 nmol

Preis (EUR)

370,00

Ambion™ CY™3 farbstoffmarkiertes synthetisches miRNA-Mimic für die miRNA-Funktionsanalyse. 5 nmol wird mitgeliefert.

• Optimiertes Design für eine korrekte Strangauslastung durch RISC
• Gereinigt und bereit für die Transfektion

Ambion™ Pre-miR™ miRNA-Vorläufer-Moleküle sind kleine, chemisch modifizierte, doppelsträngige RNA-Moleküle, die zur Nachahmung endogener reifer miRNAs entwickelt wurden. Dieses gebrauchsfertige miRNA Mimic kann mit ähnlichen Transfektions- oder Elektroporationsparametern wie bei siRNAs in Zellen eingesetzt werden. Das ermöglicht eine genaue Analyse der biologischen Wirkung von miRNA mithilfe von Funktionsexperimenten. Die Cy™3 Farbstoff-markierte Pre-miR™ Negativkontrolle Nr. 1 ist ein Pre-miR™ Molekül mit zufälliger Sequenz, das ausgiebig in humanen Zelllinien und Geweben getestet und die nachweislich keine erkennbaren Auswirkungen auf die bekannten miRNA-Funktionen hat. Ideal für die Überwachung der Transfektionseffizienz, da die Fluoreszenzmarkierung die direkte Beobachtung der zellulären Aufnahme, Verteilung und Lokalisierung der Kontrolle ermöglicht. Die Markierung befindet sich am 5'-Ende des Moleküls, und die Oligonukleotid-Sequenz ist die gleiche wie die Pre-miR™ Negativkontrolle Nr. 1 für miRNA-Vorläufermoleküle (SKU-Nr. AM17110). HINWEIS: Pre-miR™ miRNA-Vorläufer sind keine Haarnadelkonstrukte und sollten nicht mit Pre-miRNAs verwechselt werden.

Nur für Forschungszwecke. Darf nicht für diagnostische Verfahren eingesetzt werden.

Specifications

Zur Verwendung mit (Anwendung)miRNA-Analyse und Analyse nicht-codierender RNA

Marker oder FarbstoffCy3

ProduktlinieAmbion, Cy3, Pre-miR

ProdukttypmiRNA-Inhibitor

ReinheitHPLC

Menge5 nmol

VersandbedingungTrockeneis

RegelungsartNegativkontrolle

FormatRörchen

RNAi TypemiRNA Mimic

Unit SizeEach

Inhalt und Lagerung

Pre-miR™ miRNA wird zusammen mit nukleasefreiem Wasser für die Resuspension in einem einzelnen Röhrchen getrocknet geliefert und sollte bei –20 °C gelagert werden. Die Haltbarkeit ist für 1 Jahr garantiert, wenn es bei –20 °C getrocknet gelagert wird.

Häufig gestellte Fragen (FAQ)

Where can I find more information about published miRNA sequences and annotation?

Please visit miRBase: The microRNA database (http://www.mirbase.org/).

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

I would like to order a mimic or inhibitor with a 5' label. Can you do this?

We can label our Pre-miR and Anti-miR miRNA, but do not offer labeled mirVana Mimics and inhibitors. 5' modifications include Alexa Fluor 555, Alexa Fluor 647, FAM, and Cy3. Request a quote here (http://www.thermofisher.com/us/en/home/global/forms/custom-sirna-mirna-quote-request.html).

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

The miRNA sequence I need to target is not in the miRBase database. Can I order a custom mimic or inhibitor?

Yes, submit your unpublished miRNA sequences and/or order custom modified miRNA here (http://www.thermofisher.com/order/custom-genomic-products/tools/mirna/).

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

What is the difference between Invitrogen Anti-miR inhibitors & Invitrogen Pre-miR miRNA Precursors and mirVana miRNA mimics and inhibitors?

Invitrogen Anti-miR inhibitors & Invitrogen Pre-miR miRNA Precursors are our first generation mimic and inhibitors. Invitrogen Anti-miR miRNA Inhibitors are chemically modified, single stranded nucleic acids designed to specifically bind and inhibit endogenous microRNA (miRNA) molecules. These are ready to use inhibitors that can be introduced into cells using transfection or electroporation. Pre-designed inhibitors have been created for all miRNAs listed in miRBase version 15.0.
Invitrogen Pre-miR miRNA Precursors are small, chemically modified double-stranded RNA molecules designed to mimic endogenous mature miRNAs. These ready-to-use miRNA mimics, which are similar to, but not identical to siRNAs, can be introduced into cells using transfection or electroporation parameters similar to those used for siRNAs. Please note, Pre-miR miRNA Precursors are not hairpin constructs and should not be confused with pre-miRNAs.
mirVana miRNA mimics and inhibitors are our second generation of mimics and inhibitors. mirVana miRNA mimics are small, chemically double-stranded RNAs that mimic endogenous mature miRNAs and enable miRNA functional analysis by up-regulation of miRNA activity. They have enhanced specificity over the first generation mimics, are delivered as HPLC purified, and ready to transfect.
miRNA inhibitors are small, chemically modified single-stranded RNA molecules designed to specifically bind to and inhibit endogenous miRNA molecules and enable miRNA functional analysis by down-regulation of miRNA activity. They have enhanced potency over our first generation inhibitors. Pre-designed inhibitors were created for all miRNAs listed in miRBase version 19.0. They come delivered as HPLC purified, and ready to transfect. Learn more about mirVana miRNA mimics and inhibitors through this video (https://www.youtube.com/watch?feature=player_embedded&v=Q3MO1lmT0kw). Both mimics and inhibitors are available individually or as libraries.
They are compatible with both in vitro and in vivo applications, and have been validated with Lipofectamine RNAiMAX Transfection Reagent for use in cell-based systems, and with Invivofectamine 3.0 Transfection Reagent for in vivo delivery. These oligonucleotides are nontoxic and do not induce an immune response in the animal models tested. In vivo-ready mirVana miRNA mimics and inhibitors have been purified by HPLC and dialysis and are ready for immediate in vivo use. For all library inquiries, please email: rnailibraries@lifetech.com.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

What is the difference between siRNA and miRNA?

siRNA mediates degradation of a target mRNA molecule in a sequence-specific manner, while miRNA suppresses translation without cleaving mRNA.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

View all Cy3™ Dye-Labeled Pre-miR Negative Control #1 FAQs