The Ambion® QuantumRNA™ 18S Internal Standard with Competimer™ technology uses specially modified Competimer™s of the same sequence as the normal 18S primers that cannot be extended. By adjusting the ratio of 18S Competimer™s to normal 18S mRNA primers, the signal for 18S mRNA can be attenuated to the level of very rare messages. They are used for quantitative end-point RT-PCR and include sufficient reagents for 100 reactions. Because of its invariant expression across tissues and treatments, 18S ribosomal RNA is an ideal internal control for quantitative RNA analysis. The Classic 18S Internal Standards contain the same primers/Competimer™s that were part of the original QuantumRNA™ 18S Internal Standards Module. They produce a 489 bp PCR product.
Accessory Products: The QuantumRNA™ Classic II 18S Internal Standard (SKU# AM1717) produce a 324 bp PCR product, and the QuantumRNA™ Universal 18S Internal Standard (SKU# AM1718) function across the broadest range of eukaryotes, including animals, plants, and many protozoa and produce a 315 bp PCR product.
For Research Use Only. Not for use in diagnostic procedures.
Total RNA from Mouse Liver
Control Primers & Template(s)
Label or Dye
No. of Reactions
Contents & Storage
18S Primer Pair and 18S Competimer™s are stored at –20°C. Control Template RNA should be stored below –70°C. Nuclease-free water may be stored at any temperature.
Why do I need to be in linear range when analyzing 2 products in relative RT-PCR?
Ambion's QuantumRNA™ Technology Allows Multiplex Quantitative RT-PCR with 18S rRNA as an Internal Control