DNAclear™ Purification Kit

Catalog number:  AM1756

 Related applications:

DNA Extraction

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The Ambion® DNAclear™ kit is designed to purify double-stranded DNA (dsDNA) generated by enzymatic reactions such as cDNA synthesis and PCR. Includes sufficient reagents for 20 purifications.

• Efficiently purify and concentrate dsDNA ranging from 1 ng to 3 µg
• Ideal for downstream applications such as in vitro transcription
• Simple and fast protocol—about 10 minutes

The DNAclear™ cDNA Purification Kit process is simple and fast. DNA amounts ranging from 1 ng to 3 µg can be efficiently purified and concentrated. The DNAclear™ procedure consists of three steps: 1) DNA is bound to the Microfilter Cartridge in the presence of the DNA Binding Buffer, 2) the DNA is washed with the cDNA Wash Buffer, and 3) the DNA is eluted with preheated nuclease-free water. The DNAclear™ Kit can be used to remove free nucleotides, oligonucleotides, proteins, and salts from dsDNA samples. The DNA that is recovered from the Microfilter Cartridge is ready for any application that requires highly pure DNA including in vitro transcription reactions.
For Research Use Only. Not for use in diagnostic procedures.


High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Sample Type (General): Enzymatic Reactions (e.g. PCR)
Downstream Application: Cloning, In Vitro Transcription, PCR, Sequencing
Final Product: DNA
For Use With (Equipment): Centrifuge
Format: 6 microtubes
Isolation Technology: Silica Spin Column
Number of Reactions: 20 Reactions
Product Line: Ambion™, DNAclear™
Product Size: 20 preps
Purification Time: 10 min
Sample Type (Specific): PCR Products
Scale: Mini
Starting Material (Amount): 1 ng to 3 µg
Yield (up to): 3 µg
Shipping Condition: Room Temperature

Contents & storage

cDNA Binding Buffer, cDNA Wash Buffer, Microfilter Cartridges + Collection Tubes, and Elution Tubes should be stored at room temperature. Nuclease-free water may be stored at any temperature.


Manuals & protocols