RNAqueous™-96 Automated Kit

The Ambion™ RNAqueous™-96 Automated Kit is used for automated, phenol-free total RNA isolation from 100-500,000 cells or 0.1–1 mg tissue자세히 알아보기

카탈로그 번호	수량
AM1812	384 Preps

카탈로그 번호 AM1812

제품 가격(KRW)

2,092,000

카트에 추가하기

384 Preps

제품 가격(KRW)

2,092,000

카트에 추가하기

The Ambion™ RNAqueous™-96 Automated Kit is used for automated, phenol-free total RNA isolation from 100-500,000 cells or 0.1–1 mg tissue using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. The kit contains enough reagents for 384 (4 x 96) RNA purifications. The RNAqueous™-96 Automated Kit uses an RNA-binding glass-fiber filter to provide high, reproducible yields of intact RNA. An optional on-the-filter DNase treatment can be performed to ensure removal of genomic DNA for RT-PCR applications. Downloadable protocols for the use of this kit with specific liquid handling systems are available at our Automation Resource.

For Research Use Only. Not for use in diagnostic procedures.

용리량105 μL

최종 제품 유형Total RNA

용도(애플리케이션)RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling

용도(장비)MultiPROBE™ II HT, Biomek™ 2000, Liquid Handling Systems

고처리량 호환성High-throughput Compatible

수량384 Preps

배송 조건Box 1: Dry Ice
Box 2: Room Temperature

시작 물질 양Cells: ≤10⁵
Tissue: 0.1–1 mg

Isolation TechnologyVacuum Filter Plate (Glass Fiber Filter)

샘플 종류Cells, Tissue

Unit SizeEach

구성 및 보관

• 4 Filter Plates; store at room temperature
• 4 Collection Plates with lids; room temperature
• 4 Culture Plates; room temperature
• 1 Heat Plate with Lid; room temperature
• 100 mL Nuclease-free Water; room temperature
• 100 mL Elution Solution; room temperature
• 100 mL Lysis/Binding Solution; 4°C
• 100 mL Rebinding Mix; 4°C
• 2 x 175 mL Wash Solution (concentrated); 4°C
• 1.25 mL DNase 1; -20°C
• 12 mL DNase 1 Buffer; -20°C

자주 묻는 질문(FAQ)

What are the differences in the RNAqueous kits you offer?

RNaqueous kits are based on a rapid, filter-based RNA isolation system that does not require phenol, chloroform, or other toxic organic chemicals. The RNAqueous Total RNA Isolation Kit is designed for sample sizes of 10-75 mg of tissue or 10^6 to 10^7 cells, whereas the RNAqueous-Midi Total RNA Isolation Kit is designed for tissue samples of up to 0.5 g or 10^9 cells. The RNAqueous-Micro Total RNA Isolation Kit is optimized for the purification of total RNA from micro-sized samples such as 10-500,000 cultured cells, as few as 10 microdissected cells, or up to 10 mg tissue. The RNAqueous-96 Total RNA Isolation Kit utilizes a 96-well plate format for high-throughput RNA isolation from 100 to 2 x 10^6 cells or 0.1 to 1.5 mg of tissue. The RNAqueous-4PCR Total RNA Isolation Kit is designed for RNA isolation from small tissue samples (0.5-75 mg) and small- to medium-sized cultured cell samples (approximately 100-10^7 cells).