RNAqueous®-96 Automated Kit

Catalog number: AM1812

Ambion™  Related applications: Automated Nucleic Acid Purification | RNA Extraction

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The Ambion® RNAqueous®-96 Automated Kit is used for automated, phenol-free total RNA isolation from 100-500,000 cells or 0.1–1 mg tissue using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. The kit contains enough reagents for 384 (4 x 96) RNA purifications. The RNAqueous®-96 Automated Kit uses an RNA-binding glass-fiber filter to provide high, reproducible yields of intact RNA. An optional on-the-filter DNase treatment can be performed to ensure removal of genomic DNA for RT-PCR applications. Downloadable protocols for the use of this kit with specific liquid handling systems are available at our Automation Resource.
For Research Use Only. Not for use in diagnostic procedures.


Sample Type (General): Cells, Tissue
High Throughput Compatibility: Automated Protocols, Not High Throughput-Compatible (Manual), High Throughput-Compatible
Downstream Application: Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction, Northern Blotting
Format: 96-well plate
Product Size: 384 preps
Final Product: Total RNA
Isolation Technology: Vacuum Filter Plate (Glass Fiber Filter)
Sample Type (Specific): Mammalian Cells, Tissue (Mammalian)
For Use With (Equipment): Biomek® 2000, MultiPROBE® II HT
Starting Material (Amount): Up to 5 x 10^5 cells, Up to 1.0 mg tissue

Contents & storage

Store Filter Plates (4), Collection Plate with lids (4), Culture Plates (4), Heat Plate with lid (1), Nuclease-free Water (100 mL), and Elution Solution (100 mL) at room temperature. Store Lysis⁄Binding Solution (100 mL), Rebinding Mix (100 mL) and Wash Solution Concentrate (2 x 175 mL) at 4°C. Store DNase I (1.25 mL; 20U⁄µL) and DNase 1 Buffer (12 mL) at -20°C.


Manuals & protocols

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