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MagMAX™-96 Total RNA Isolation Kit

Catalog number: AM1830

Ambion™  Related applications: Automated Nucleic Acid Purification | RNA Extraction

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Description

The MagMAX™-96 Total RNA Isolation Kit is designed for the rapid, moderate-throughput purification of RNA from animal and plant cells and tissue. RNA purified using the kit is ideal for PCR and RT-PCR applications. The kit uses magnetic bead–based purification and includes sufficient reagents for 96 reactions. Features of the MagMAX™-96 Total RNA Isolation Kit include:

• Enhanced lysis/binding conditions to accommodate larger sample inputs
• Reagents for TURBO DNase™ treatment included to minimize DNA contamination and preserve RNA integrity
• Includes protocol for RNA isolation from plant tissues
• 96 reactions per kit suitable for moderate throughput users

Benefits of MagMAX™ magnetic bead–based purification
Magnetic beads offer many benefits compared to other technologies for isolating RNA. Beads bind RNA more efficiently than glass fiber filters, resulting in higher and more consistent RNA yields. Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples. Since only a small volume of magnetic beads is needed for high-efficiency binding, the bound RNA can be eluted in just 20–50 µL of nuclease-free water, concentrating RNA from large, dilute samples.

Accommodates diverse sample types
The kit accommodates diverse biological samples and a broad range of cell and tissue input amounts. The kit is optimized for high-throughput isolation of RNA from 25 cells to 2 x 106 cells, as well as small plant and mammalian tissue samples (up to 10 mg). The protocol is fully amenable to automation. Detailed guidelines for general automation are included with the kit, and downloadable protocols for the use of this kit with specific liquid handling systems are available at the Automation Resource.

Accessory products
Lysis/Binding Solution Concentrate (AM8500), Wash Solution 1 Concentrate (AM8504), and Wash Solution 2 Concentrate (AM8640) are components of the kit that may also be purchased separately. The Plant RNA Isolation Aid (AM9690) can be used in conjunction with the MagMAX™-96 Total RNA Isolation Kit for the isolation of RNA from plant tissues.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): Cells, Tissue, Plant Samples
High Throughput Compatibility: Automated Protocols, High Throughput-Compatible, Not High Throughput-Compatible (Manual)
Downstream Application: Reverse Transcriptase PCR (RT-PCR), Microarray Analysis, cDNA Library Construction, Real-Time Quantitative PCR (qPCR), Northern Blotting, Nuclease Protection Assays
Format: 96-well plate
Product Size: 96 preps
Final Product: Total RNA
Yield (up to): 40 µg
Isolation Technology: Magnetic Bead
Sample Type (Specific): Mammalian Cells, Plant, Tissue (Mammalian)
For Use With (Equipment): MagMAX™ Express-96 Standard Magnetic Particle Processor, Robotic Liquid Handling Systems
Starting Material (Amount): Up to 10 mg plant tissue, Up to 2 x10^6 cells, Up to 5 mg animal tissue

Contents & storage

Kit contents (storage):
• 1 Processing Plate & Lid (room temperature)
• 11 ml Lysis/Binding Solution Concentrate (room temperature)
• 18 ml Wash Solution 1 Concentrate (room temperature)
• 55 ml Wash Solution 2 Concentrate (room temperature)
• 12 ml RNA Rebinding Concentrate (room temperature)
• 10 ml Elution Buffer (4°C or room temperature)
• 6 ml MagMAX™ TURBO™ DNase Buffer (4°C or room temperature)
• 1.1 ml RNA Binding Beads (4°C)
• 1.1 ml Lysis/Binding Enhancer (-20°C)
• 110 µl TURBO™ DNase (-20°C)

Contains enough reagents to isolate RNA from 96 samples.

Documents

Manuals & protocols

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