MEGAclear™ Transcription Clean-Up Kit

Description

The MEGAclear™ Kit is designed for the quick cleanup of any large-scale transcription reaction. The MEGAclear™ Kit efficiently separates RNA from unincorporated NTPs, enzymes, and buffer components. Advantages of the MEGAclear™ Kit:

• Purifies transcription reactions to produce high-quality RNA, free of unincorporated NTPs, enzymes, and buffer components
• High recovery from 1 ng to 500 µg of input
• Ideal for purification of aRNA amplification reactions, nonisotopically labeled RNA probes, and capped RNA transcripts for oocyte injections
• Quick protocol—purify RNA in less than 30 minutes

Using the MEGAclear™ Kit
The protocol is quick and easy. The transcription reaction is mixed with Binding Solution, passed over an RNA-binding filter, and washed; an elution step releases the purified RNA, which is now ready for any application requiring highly pure synthetic or in vitrotranscribed RNA. For RNA recovery of 70% or greater, single-stranded RNA should be >100 nt and double-stranded RNA should be >200 bp.

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Specifications

Sample Type (General):	RNA
High Throughput Compatibility:	Not High Throughput-Compatible (Manual)
Downstream Application:	Northern Blotting, Nuclease Protection Assays
Final Product:	In Vitro Transcribed RNA (Purified)
For Use With (Equipment):	Centrifuge, Vacuum Manifold
Format:	Tube(s)
Isolation Technology:	Silica Spin Column
Number of Reactions:	20 Preps
Product Line:	Ambion™, MEGAclear™
Product Size:	20 preps
Purification Time:	30 min
Sample Type (Specific):	In Vitro Transcribed RNA, Total RNA
Starting Material (Amount):	1 ng to 500 µg of dsRNA>200b or ssRA>100bp
Yield (up to):	500 µg
Shipping Condition:	Room Temperature

Contents & storage