MEGAclear™ Transcription Clean-Up Kit

Catalog number: AM1908

Ambion™  Related applications: In Vitro Transcription

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

The MEGAclear™ Kit is designed for the quick cleanup of any large-scale transcription reaction. The MEGAclear™ Kit efficiently separates RNA from unincorporated NTPs, enzymes, and buffer components. Advantages of the MEGAclear™ Kit:

• Purifies transcription reactions to produce high-quality RNA, free of unincorporated NTPs, enzymes, and buffer components
• High recovery from 1 ng to 500 µg of input
• Ideal for purification of aRNA amplification reactions, nonisotopically labeled RNA probes, and capped RNA transcripts for oocyte injections
• Quick protocol—purify RNA in less than 30 minutes

Using the MEGAclear™ Kit
The protocol is quick and easy. The transcription reaction is mixed with Binding Solution, passed over an RNA-binding filter, and washed; an elution step releases the purified RNA, which is now ready for any application requiring highly pure synthetic or in vitrotranscribed RNA. For RNA recovery of 70% or greater, single-stranded RNA should be >100 nt and double-stranded RNA should be >200 bp.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): RNA
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Downstream Application: Northern Blotting, Nuclease Protection Assays
Format: Tube(s)
Product Size: 20 preps
Final Product: In Vitro Transcribed RNA (Purified)
Yield (up to): 500 µg
Purification Time: 30 min
Number of Reactions: 20 Preps
Isolation Technology: Silica Spin Column
Sample Type (Specific): Total RNA, In Vitro Transcribed RNA
For Use With (Equipment): Centrifuge, Vacuum Manifold
Starting Material (Amount): 1 ng to 500 µg of dsRNA>200b or ssRA>100bp
Shipping Condition: Room Temperature

Contents & storage

Binding Solution, Wash Solution Concentrate, and 5 M Ammonium Acetate are stored at 4°C. Elution Solution can be stored at any temperature, and Filter Cartridges and Collection and Elution Tubes are stored at room temperature.

Documents

Manuals & protocols

Recommended products