MEGAclear™ Transcription Clean-Up Kit

Catalog number:  AM1908

 Related applications:

In Vitro Transcription

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The MEGAclear™ Kit is designed for the quick cleanup of any large-scale transcription reaction. The MEGAclear™ Kit efficiently separates RNA from unincorporated NTPs, enzymes, and buffer components. Advantages of the MEGAclear™ Kit:

• Purifies transcription reactions to produce high-quality RNA, free of unincorporated NTPs, enzymes, and buffer components
• High recovery from 1 ng to 500 µg of input
• Ideal for purification of aRNA amplification reactions, nonisotopically labeled RNA probes, and capped RNA transcripts for oocyte injections
• Quick protocol—purify RNA in less than 30 minutes

Using the MEGAclear™ Kit
The protocol is quick and easy. The transcription reaction is mixed with Binding Solution, passed over an RNA-binding filter, and washed; an elution step releases the purified RNA, which is now ready for any application requiring highly pure synthetic or in vitrotranscribed RNA. For RNA recovery of 70% or greater, single-stranded RNA should be >100 nt and double-stranded RNA should be >200 bp.
For Research Use Only. Not for use in diagnostic procedures.


Sample Type (General): RNA
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Downstream Application: Northern Blotting, Nuclease Protection Assays
Final Product: In Vitro Transcribed RNA (Purified)
For Use With (Equipment): Centrifuge, Vacuum Manifold
Format: Tube(s)
Isolation Technology: Silica Spin Column
Number of Reactions: 20 Preps
Product Line: Ambion™, MEGAclear™
Product Size: 20 preps
Purification Time: 30 min
Sample Type (Specific): In Vitro Transcribed RNA, Total RNA
Starting Material (Amount): 1 ng to 500 µg of dsRNA>200b or ssRA>100bp
Yield (up to): 500 µg
Shipping Condition: Room Temperature

Contents & storage

Binding Solution, Wash Solution Concentrate, and 5 M Ammonium Acetate are stored at 4°C. Elution Solution can be stored at any temperature, and Filter Cartridges and Collection and Elution Tubes are stored at room temperature.


Manuals & protocols