Invitrogen™
MEGAclear™ Transcription Clean-Up Kit
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Description
The MEGAclear™ Kit is designed for the quick cleanup of any large-scale transcription reaction. The MEGAclear™ Kit efficiently separates RNA from unincorporated NTPs, enzymes, and buffer components. Advantages of the MEGAclear™ Kit:• Purifies transcription reactions to produce high-quality RNA, free of unincorporated NTPs, enzymes, and buffer components
• High recovery from 1 ng to 500 µg of input
• Ideal for purification of aRNA amplification reactions, nonisotopically labeled RNA probes, and capped RNA transcripts for oocyte injections
• Quick protocol—purify RNA in less than 30 minutes
Using the MEGAclear™ Kit
The protocol is quick and easy. The transcription reaction is mixed with Binding Solution, passed over an RNA-binding filter, and washed; an elution step releases the purified RNA, which is now ready for any application requiring highly pure synthetic or in vitrotranscribed RNA. For RNA recovery of 70% or greater, single-stranded RNA should be >100 nt and double-stranded RNA should be >200 bp.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Sample Type (General): | RNA |
|---|---|
| High Throughput Compatibility: | Not High Throughput-Compatible (Manual) |
| Downstream Application: | Northern Blotting, Nuclease Protection Assays |
| Final Product: | In Vitro Transcribed RNA (Purified) |
| For Use With (Equipment): | Centrifuge, Vacuum Manifold |
| Format: | Tube(s) |
| Isolation Technology: | Silica Spin Column |
| Number of Reactions: | 20 Preps |
| Product Line: | Ambion™, MEGAclear™ |
| Product Size: | 20 preps |
| Purification Time: | 30 min |
| Sample Type (Specific): | In Vitro Transcribed RNA, Total RNA |
| Starting Material (Amount): | 1 ng to 500 µg of dsRNA>200b or ssRA>100bp |
| Yield (up to): | 500 µg |
| Shipping Condition: | Room Temperature |
Contents & storage
Binding Solution, Wash Solution Concentrate, and 5 M Ammonium Acetate are stored at 4°C. Elution Solution can be stored at any temperature, and Filter Cartridges and Collection and Elution Tubes are stored at room temperature.
