Invitrogen™

MEGAclear™-96 Transcription Clean-Up Kit

Catalog number: AM1909
Invitrogen™

MEGAclear™-96 Transcription Clean-Up Kit

Catalog number: AM1909
Catalog Number
AM1909
Unit Size
96 preps
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
AM190996 preps
-
Product Overview
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The MEGAclear™-96 Kit is designed for the quick, high-throughput cleanup of any large-scale transcription reaction. The kit efficiently separates RNA from unincorporated NTPs, enzymes, and buffer components.

• Purifies transcription reactions in a 96-well format to produce high quality RNA free of unincorporated NTPs, enzymes, and buffer components
• Quick protocol—purify RNA in less than 20 minutes
• Can be adapted to robotic platforms

The MEGAclear™-96 Kit offers more consistent results and higher recovery than generic RNA isolation methods that have been adapted for RNA clean-up. The protocol is quick and easy. The sample is mixed with Binding Solution and ethanol, transferred to a 96-well filter plate, and washed twice; an elution step releases purified RNA, ready for any application requiring highly pure synthetic or in vitro-transcribed RNA. The MEGAclear-96 Kit can also be integrated with your robotic platform or used with manual methods.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Final Product Type
Purified Synthetic mRNA, Purified mRNA
Format
96-well Plate
Isolation Technology
Silica Spin Column
Purification Target
mRNA, Synthetic mRNA
Sample Type
mRNA, Synthetic mRNA
For Use With (Application)
In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
For Use With (Equipment)
Vacuum Manifold, Centrifuge
High-throughput Compatibility
High-throughput Compatible
No. of Reactions
96 Preps
Product Line
Ambion™, MEGAclear™
Product Type
mRNA purification kit, Purification kit
Quantity
96 Preps
Purification Time
30 min
Shipping Condition
Room Temperature
Starting Material Volume
1 ng to 500 μg of dsRNA>200b or ssRA>100bp
Yield
100 μg
Starting Material
synthetic mRNA, mRNA

Contents & Storage

• 8 ml Binding Solution (store at 4°C)
• 20 ml Wash Solution Concentrate (store at 4°C)
• 10 ml Elution Solution (store at room temperature, 4°C, or -20°C)
• 20 ml Nuclease-free Water (store at room temperature, 4°C, or -20°C)
• 1 MEGAclear™-96 Filter Plate (store at room temperature)
• 1 Collection Plate (store at room temperature)
• 2 Adhesive Plate Sealers (store at room temperature)

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