MEGAclear™-96 Transcription Clean-Up Kit

Catalog number: AM1909

Ambion™  Related applications: In Vitro Transcription

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The MEGAclear™-96 Kit is designed for the quick, high-throughput cleanup of any large-scale transcription reaction. The kit efficiently separates RNA from unincorporated NTPs, enzymes, and buffer components.

• Purifies transcription reactions in a 96-well format to produce high quality RNA free of unincorporated NTPs, enzymes, and buffer components
• Quick protocol—purify RNA in less than 20 minutes
• Can be adapted to robotic platforms

The MEGAclear™-96 Kit offers more consistent results and higher recovery than generic RNA isolation methods that have been adapted for RNA clean-up. The protocol is quick and easy. The sample is mixed with Binding Solution and ethanol, transferred to a 96-well filter plate, and washed twice; an elution step releases purified RNA, ready for any application requiring highly pure synthetic or in vitro-transcribed RNA. The MEGAclear-96 Kit can also be integrated with your robotic platform or used with manual methods.
For Research Use Only. Not for use in diagnostic procedures.


Sample Type (General): RNA
High Throughput Compatibility: High Throughput-Compatible
Downstream Application: Northern Blotting, Nuclease Protection Assays, Microarray Analysis
Format: 96-well plate
Product Size: 96 preps
Final Product: In Vitro Transcribed RNA (Purified)
Yield (up to): 100 µg
Purification Time: 30 min
Number of Reactions: 96 Preps
Isolation Technology: Silica Spin Column
Sample Type (Specific): Total RNA, In Vitro Transcribed RNA
For Use With (Equipment): Centrifuge, Vacuum Manifold
Starting Material (Amount): 1 ng to 500 µg of dsRNA>200b or ssRA>100bp
Shipping Condition: Room Temperature

Contents & storage

• 8 ml Binding Solution (store at 4°C)
• 20 ml Wash Solution Concentrate (store at 4°C)
• 10 ml Elution Solution (store at room temperature, 4°C, or -20°C)
• 20 ml Nuclease-free Water (store at room temperature, 4°C, or -20°C)
• 1 MEGAclear™-96 Filter Plate (store at room temperature)
• 1 Collection Plate (store at room temperature)
• 2 Adhesive Plate Sealers (store at room temperature)


Manuals & protocols