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Invitrogen™
MEGAclear™-96 Transcription Clean-Up Kit
The MEGAclear™-96 Kit is designed for the quick, high-throughput cleanup of any large-scale transcription reaction. The kit efficiently separates RNARead more
| Catalog Number | Quantity |
|---|---|
| AM1909 | 96 Preps |
Catalog number AM1909
Price (MXN)
-
Quantity:
96 Preps
The MEGAclear™-96 Kit is designed for the quick, high-throughput cleanup of any large-scale transcription reaction. The kit efficiently separates RNA from unincorporated NTPs, enzymes, and buffer components.
• Purifies transcription reactions in a 96-well format to produce high quality RNA free of unincorporated NTPs, enzymes, and buffer components
• Quick protocol—purify RNA in less than 20 minutes
• Can be adapted to robotic platforms
The MEGAclear™-96 Kit offers more consistent results and higher recovery than generic RNA isolation methods that have been adapted for RNA clean-up. The protocol is quick and easy. The sample is mixed with Binding Solution and ethanol, transferred to a 96-well filter plate, and washed twice; an elution step releases purified RNA, ready for any application requiring highly pure synthetic or in vitro-transcribed RNA. The MEGAclear-96 Kit can also be integrated with your robotic platform or used with manual methods.
• Purifies transcription reactions in a 96-well format to produce high quality RNA free of unincorporated NTPs, enzymes, and buffer components
• Quick protocol—purify RNA in less than 20 minutes
• Can be adapted to robotic platforms
The MEGAclear™-96 Kit offers more consistent results and higher recovery than generic RNA isolation methods that have been adapted for RNA clean-up. The protocol is quick and easy. The sample is mixed with Binding Solution and ethanol, transferred to a 96-well filter plate, and washed twice; an elution step releases purified RNA, ready for any application requiring highly pure synthetic or in vitro-transcribed RNA. The MEGAclear-96 Kit can also be integrated with your robotic platform or used with manual methods.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products
For Use With (Equipment)Vacuum Manifold, Centrifuge
High-throughput CompatibilityHigh-throughput Compatible
No. of Reactions96 Preps
Product LineAmbion, MEGAclear
Product TypemRNA purification kit, Purification kit
Purification Time30 min
Quantity96 Preps
Shipping ConditionRoom Temperature
Starting Materialsynthetic mRNA, mRNA
Starting Material Volume1 ng to 500 μg of dsRNA>200b or ssRA>100bp
Yield100 μg
Final Product TypePurified Synthetic mRNA, Purified mRNA
Format96-well Plate
Isolation TechnologySilica Spin Column
Purification TargetmRNA, Synthetic mRNA
Sample TypemRNA, Synthetic mRNA
Unit SizeEach
Contents & Storage
• 8 ml Binding Solution (store at 4°C)
• 20 ml Wash Solution Concentrate (store at 4°C)
• 10 ml Elution Solution (store at room temperature, 4°C, or -20°C)
• 20 ml Nuclease-free Water (store at room temperature, 4°C, or -20°C)
• 1 MEGAclear™-96 Filter Plate (store at room temperature)
• 1 Collection Plate (store at room temperature)
• 2 Adhesive Plate Sealers (store at room temperature)
• 20 ml Wash Solution Concentrate (store at 4°C)
• 10 ml Elution Solution (store at room temperature, 4°C, or -20°C)
• 20 ml Nuclease-free Water (store at room temperature, 4°C, or -20°C)
• 1 MEGAclear™-96 Filter Plate (store at room temperature)
• 1 Collection Plate (store at room temperature)
• 2 Adhesive Plate Sealers (store at room temperature)