RNAqueous™-Midi Total RNA Isolation Kit

Catalog number:  AM1911

 Related applications:

RNA Extraction

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The Ambion® RNAqueous®-Midi Kit is for phenol-free total RNA isolation from 0.1 g to 0.5 g of tissue or 107-108 cells using a guanidinium-based lysis/;denaturant and glass fiber filter separation technology. The RNAqueous®-Midi Kit provides enough reagents for 15 RNA purifications.
• Convenient syringe-based filtration
• Compatible with vacuum manifolds when processing multiple samples The Ambion® RNAqueous®-Midi Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.
WARNING: Cancer and Reproductive Harm –
For Research Use Only. Not for use in diagnostic procedures.


Sample Type (General): Cells, Tissue
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Downstream Application: Northern Blotting, Nuclease Protection Assays, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction
Final Product: Total RNA
Isolation Technology: Syringe Filter (Glass Fiber)
Product Line: Ambion™, RNAqueous®
Product Size: 15 preps
Shipping Condition: Room Temperature

Contents & storage

Formaldehyde Load Dye should be stored at -20°C; Lysis/Binding Solution, Water for 64% Ethanol, Lithium Chloride Solution, Wash Solution #1 and Wash Solution 2/3 Concentrate should be stored at 4°C; Syringe Filter Units, Collection Tubes and Elution Solution can be stored at room temperature. All reagents are guaranteed stable for 6 months when properly stored.


Manuals & protocols