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Invitrogen™
RNAqueous™ Total RNA Isolation Kit
Catalog number: AM1912
Invitrogen™
RNAqueous™ Total RNA Isolation Kit
Catalog number: AM1912
Catalog Number
AM1912
Unit Size
50 preps
Price (USD)
Price: 411.00
Your Price:
Availability
-
Quantity
| Catalog Number | Unit Size | Price (USD) | Availability | Quantity | |
|---|---|---|---|---|---|
| AM1912 | 50 preps | Price: 411.00 Your Price: | - |
Recurring order eligible
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Product Overview
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Additional Information
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The RNAqueous™ Kit is for phenol-free total RNA isolation from 1–75 mg of tissue or from 102–107 cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. The RNAqueous™ Kit provides:
• Convenient syringe-based filtration
• Compatibility with vacuum manifolds when processing multiple samples
How it works
The RNAqueous™ Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.
• Convenient syringe-based filtration
• Compatibility with vacuum manifolds when processing multiple samples
How it works
The RNAqueous™ Kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.
WARNING: Cancer and Reproductive Harm – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Isolation Technology
Spin Column (Glass Fiber Filter)
Elution Volume
50–120 μL
Sample Type
Bacteria, Cells, Plant, Tissue, Yeast, Viruses, Enzymatic Reactions
Final Product Type
Total RNA
High-throughput Compatibility
Not High-throughput Compatible (Manual)
For Use With (Application)
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Quantity
50 Preps
Shipping Condition
Room Temperature
Starting Material Amount
Bacteria: ≤109 cells
Cells: ≤107
Tissue: ≤75 mg
Plant: ≤75 mg
Yeast: ≤108 cells
Virus: varies
Enzymatic Reactions: varies
Cells: ≤107
Tissue: ≤75 mg
Plant: ≤75 mg
Yeast: ≤108 cells
Virus: varies
Enzymatic Reactions: varies
Contents & Storage
• 6 mL Formaldehyde Load Dye; store at -20°C
• 60 mL Lysis/Binding Solution; 4°C
• 60 mL Water; 4°C
• 4 mL Lithium Chloride Precipitation Solution; 4°C
• 40 mL Wash Solution #1; 4°C
• 80 mL Wash Solution #2/3 (concentrated); 4°C
• 50 Filter Cartridges; room temperature
• 100 Collection Tubes; room temperature
• 10 mL Elution Solution; room temperature
• 60 mL Lysis/Binding Solution; 4°C
• 60 mL Water; 4°C
• 4 mL Lithium Chloride Precipitation Solution; 4°C
• 40 mL Wash Solution #1; 4°C
• 80 mL Wash Solution #2/3 (concentrated); 4°C
• 50 Filter Cartridges; room temperature
• 100 Collection Tubes; room temperature
• 10 mL Elution Solution; room temperature