RNAqueous®-4PCR Total RNA Isolation Kit

Catalog number: AM1914

Ambion™  Related applications: RNA Extraction

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The RNAqueous®-4PCR Kit is used for phenol-free total RNA isolation from small tissue samples (0.5 mg–75 mg) or from cells (~100 cells to ~1 x 107 cells), using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. DNA-free™ DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR. Features of this kit:

• DNA-free RNA for RT-PCR
• Rapid, phenol-free total RNA isolation
• Novel reagent for rapid and safe removal of DNase and divalent cations

Using the RNAqueous®-4PCR Kit
The RNAqueous®-4PCR Kit is designed for isolatation of RNA for applications such as RT-PCR, that would benefit from its robust genomic DNA removal capabilities. When used in conjunction with RNAlater® Tissue Collection: RNA Stabilization Solution, most tissue samples can be stored at 4°C and then easily disrupted in standard microfuge tubes using the pestles provided in the kit. There is no need for snap-freezing in liquid nitrogen or grinding in a mortar and pestle.
For Research Use Only. Not for use in diagnostic procedures.


Sample Type (General): Cells, Tissue
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Downstream Application: Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction, Northern Blotting
Product Size: 30 preps
Final Product: Total RNA
Isolation Technology: Silica Spin Column

Contents & storage

• 600 µl 10X DNase I Buffer (-20°C)
• 45 µl DNase I 2 U/µl (-20°C)
• 1 ml Formaldehyde Load Dye (-20°C)
• 100 µl Linear Acrylamide 5 mg/ml (-20°C)
• 50 µl Positive Control Primers (-20°C)
• 1 ml 5M Ammonium Acetate (-20°C)
• 35 ml Water for 64% Ethanol (4°C)
• 600 ml DNase Inactivation Reagent (4°C)
• 30 ml Lysis/Binding Solution (4°C)
• 25 ml Wash Solution 1 (4°C)
• 35 ml Wash Solution 2/3 (4°C)
• 3 Pestles for tissue disruption (room temperature)
• 30 Filter Cartridges (room temperature)
• 60 Collection Tubes (room temperature)
• 25 ml Elution Solution (-20°C, 4°C, or room temperature)


Manuals & protocols

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