The RNAqueous™-4PCR Kit is used for phenol-free total RNA isolation from small tissue samples (0.5 mg–75 mg) or from cells (∼100 cells to ∼1 x 107 cells), using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. DNA-free™ DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR. Features of this kit:
• DNA-free RNA for RT-PCR • Rapid, phenol-free total RNA isolation • Novel reagent for rapid and safe removal of DNase and divalent cations
Using the RNAqueous™-4PCR Kit The RNAqueous™-4PCR Kit is designed for isolatation of RNA for applications such as RT-PCR, that would benefit from its robust genomic DNA removal capabilities. When used in conjunction with RNAlater™ Tissue Collection: RNA Stabilization Solution, most tissue samples can be stored at 4°C and then easily disrupted in standard microfuge tubes using the pestles provided in the kit. There is no need for snap-freezing in liquid nitrogen or grinding in a mortar and pestle.
For Research Use Only. Not for use in diagnostic procedures.