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RNAqueous™-4PCR Total RNA Isolation Kit

Catalog number: AM1914

RNAqueous™-4PCR Total RNA Isolation Kit

Catalog number: AM1914
Catalog Number
Unit Size
30 preps
Price (USD)
Price: 293.00
Your Price:
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
AM191430 preps
Price: 293.00
Your Price:
Product Overview
Citations & References
Additional Information
The RNAqueous™-4PCR Kit is used for phenol-free total RNA isolation from small tissue samples (0.5 mg–75 mg) or from cells (∼100 cells to ∼1 x 107 cells), using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. DNA-free™ DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR. Features of this kit:

• DNA-free RNA for RT-PCR
• Rapid, phenol-free total RNA isolation
• Novel reagent for rapid and safe removal of DNase and divalent cations

Using the RNAqueous™-4PCR Kit
The RNAqueous™-4PCR Kit is designed for isolatation of RNA for applications such as RT-PCR, that would benefit from its robust genomic DNA removal capabilities. When used in conjunction with RNAlater™ Tissue Collection: RNA Stabilization Solution, most tissue samples can be stored at 4°C and then easily disrupted in standard microfuge tubes using the pestles provided in the kit. There is no need for snap-freezing in liquid nitrogen or grinding in a mortar and pestle.
For Research Use Only. Not for use in diagnostic procedures.


Isolation Technology
Silica Spin Column
Purification Time
10 min. (After sample homogenization)
Elution Volume
50–120 μL
Sample Type
Tissue, Cells
Final Product Type
Total RNA
High-throughput Compatibility
Not High-throughput Compatible (Manual)
For Use With (Application)
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
30 Preps
Shipping Condition
Box 1: Dry Ice
Box 2: Room Temperature
Starting Material Amount
Tissue: ≤75 mg
Cells: ≤107
≤10 μg per 1 mg tissue
≤1 μg per 1 x 105 cells

Contents & Storage

• 600 µL 10X DNase 1 Buffer; store at -20°C
• 45 µL DNase 1; -20°C
• 1 mL Formaldehye Load Dye; -20°C
• 100 µL Linear Acrylamide; -20°C
• 50 µL Positive Control Primers; -20°C
• 1 mL 5M Ammonium Acetate; -20°C
• 35 mL Water; 4°C
• 600 µL DNase Inactivation Reagent; 4°C
• 30 mL Lysis/Binding Solution; 4°C
• 25 mL Wash Solution #1; 4°C
• 35 mL Wash Solution 2/3 (concentrated); 4°C
• 3 Pestles; room temperature
• 30 Filter Cartridges; room temperature
• 60 Collection Tubes; room temperature
• 25 mL Elution Solution; room temperature


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    Citations & References

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