RNAqueous®-96 Total RNA Isolation Kit

Catalog number: AM1920

Ambion™  Related applications: RNA Extraction

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Description

The RNAqueous®-96 Kit is used for high-throughput, phenol-free total RNA isolation from 100–2,000,000 cells or 0.1–1.5 mg of tissue using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. The kit contains sufficient reagents for 192 (2 x 96) RNA purifications. The RNAqueous®-96 Kit is ideal for the isolation of total RNA from multiple samples and very small samples.

Using the RNAqueous®-96 Kit
The procedure uses an RNA-binding glass-fiber filter and either vacuum filtration or centrifugation methods to provide high yields of intact RNA. An optional on-the-filter DNase treatment can be performed to ensure removal of genomic DNA for RT-PCR applications. The 96-well plates are compatible with most common 96-well plate vacuum manifolds and 96-well plate centrifuge rotors. The RNAqueous®-96 Kit provides consistently high yields of RNA from various amounts of starting material. The high quality of RNA isolated is illustrated (see figure).

Accessory product
The RNAqueous®-96 Automated Kit (Cat. No. AM1812) is the same product in a format suitable for automation.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): Cells, Tissue
High Throughput Compatibility: Automated Protocols, Not High Throughput-Compatible (Manual), High Throughput-Compatible
Downstream Application: Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction, Northern Blotting
Format: 96-well plate
Product Size: 192 preps
Final Product: Total RNA
Isolation Technology: Vacuum Filter Plate (Glass Fiber Filter)
Sample Type (Specific): Mammalian Cells, Tissue (Mammalian)
For Use With (Equipment): Vacuum Manifold (96-Well Plate), 96-Well Plate Centrifuge Rotors

Contents & storage

Store Nuclease–free Water, Elution Solution, Filter Plates, Collection Plates, Adhesive Plate Sealers, and Plate Lid at room temperature. Store Lysis⁄Binding Solution, Wash Solution Concentrate, and Rebinding Mix at 4°C. Store DNase I Buffer and DNase I at -20°C.

Documents

Manuals & protocols

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