The RNAqueous™-96 Kit is used for high-throughput, phenol-free total RNA isolation from 100–2,000,000 cells or 0.1–1.5 mg of tissue using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. The kit contains sufficient reagents for 192 (2 x 96) RNA purifications. The RNAqueous™-96 Kit is ideal for the isolation of total RNA from multiple samples and very small samples.
Using the RNAqueous™-96 Kit The procedure uses an RNA-binding glass-fiber filter and either vacuum filtration or centrifugation methods to provide high yields of intact RNA. An optional on-the-filter DNase treatment can be performed to ensure removal of genomic DNA for RT-PCR applications. The 96-well plates are compatible with most common 96-well plate vacuum manifolds and 96-well plate centrifuge rotors. The RNAqueous™-96 Kit provides consistently high yields of RNA from various amounts of starting material. The high quality of RNA isolated is illustrated (see figure).
Accessory product The RNAqueous™-96 Automated Kit (Cat. No. AM1812) is the same product in a format suitable for automation.
For Research Use Only. Not for use in diagnostic procedures.
• 50 mL Nuclease-free Water; store at room temperature • 50 mL Elution Solution; room temperature • 2 Filter Plates; room temperature • 2 Collection Plates; room temperature • 2 Adhesive Plate Sealers; room temperature • 2 Plate Lids; room temperature • 50 mL Lysis/Binding Solution; 4°C • 175 mL Wash Solution (concentrated); 4°C • 53 mL Rebinding Mix; 4°C • 12 mL DNase I Buffer; -20°C • 1.25 mL DNase I; -20°C