RiboPure™ RNA Purification Kit, bacteria

Catalog number: AM1925

Ambion™  Related applications: RNA Extraction

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Description

The Ambion® RiboPure™-Bacteria Kit provides a rapid, robust, column-based method for isolating high quality RNA from bacteria. Total RNA can be isolated from the toughest Gram positive bacteria samples thanks to the kit's comprehensive protocol that uses zirconia beads to thoroughly disrupt bacterial cell walls. The RiboPure™-Bacteria Kit protocol also eliminates the need for harsh treatments or enzymatic digestion which may alter gene expression profile.
• Obtain purified bacterial RNA suitable for nearly any downstream application
• Prepare pure RNA samples from the most difficult-to-lyse Gram positive bacteria
• Eliminate enzymatic pretreatments that might otherwise alter gene expression
• Remove contaminating gDNA with included DNA-free™ reagents

A Comprehensive and Convenient Column Purification Procedure
The RiboPure™-Bacteria Kit purifies total RNA from a wide range of bacterial species and has been extensively tested with E. coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis, Campylobacter fetus, and Rhodobacter sphaeroides.

A Streamlined and Effective Method for Bacterial RNA Extraction
The kit protocol begins with thorough cell wall disruption using of zirconia beads, followed by organic extraction of the lysate, and RNA purification on a convenient silica column. In approximately 1 hour, RNA is of superb quality can be isolated from fresh, snap-frozen, or RNAlater® solution-treated bacterial cells (Figures 1 and 2). To help ensure complete removal of genomic DNA, an optional DNA removal step requiring approximately 30 minutes of additional time, can be incorporated. When the RiboPure™-Bacteria kit is used upstream from the MICROBExpress™ Bacterial mRNA Enrichment Kit, the sensitivity of RNA-Seq experiments or array analyses can be dramatically increased (Figure 3).

Purified RNA is Suitable for Use with a Variety of Downstream Applications and Products:
The RNA purified using the Ambion® RiboPure™-Bacteria Kit is suitable for downstream applications including RNA sequencing, microarray analysis, real-time PCR (qPCR), reverse transcription qPCR (RT-qPCR), northern blotting, and cDNA library construction.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): Bacteria
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Downstream Application: Microarray Analysis, Northern Blotting, Nuclease Protection Assays, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction
Final Product: Total RNA (Bacterial)
Isolation Technology: Organic Extraction, Spin Column
Product Size: 50 preps
Starting Material (Amount): Up to 10^9 cells

Contents & storage

Store Zirconia Beads, DNase I, 10X DNase I Buffer and DNase Inactivation Reagent at -20°C. Store RNAwiz, Wash Solution 1 and Wash Solution 2⁄3 Concentrate at 4°C. Store 0.5mL Screw Cap Tubes, Filter Cartridges, Collection Tubes, and 1.5 mL Tubes (RNase–free) at room temperature.

Documents

Manuals & protocols

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