Invitrogen™
RiboPure™ RNA Purification Kit, blood
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Description
The RiboPure™-Blood Kit is for the isolation of high-quality RNA directly from whole blood. The kit combines two methods of RNA purification to efficiently remove blood components (protein, heme, genomic DNA, and RNases) that can compromise downstream procedures. The kit includes sufficient reagents for 40 RNA purifications from whole blood samples ranging from 0.3–0.5 mL each. Advantages of the RiboPure™-Blood Kit:• Purify total RNA directly from whole blood—without prior recovery of WBCs
• Simple, fast procedure—from blood to RNA in less than 30 minutes
• Includes RNAlater® Solution—store blood samples 3 days or more at room temperature
• Includes DNA-free™ Reagents—to ready RNA for RT-PCR and array analysis
Applications
The RiboPure™-Blood Kit produces high yields of pure RNA directly from whole blood, eliminating the need to isolate WBCs prior to RNA purification. RNA recovered using the RiboPure™-Blood Kit can be used in the most challenging applications, including real-time RT-PCR and array analysis. Average yields of RNA are between 2–4 µg/0.5 mL of whole blood (variation is due to differences in WBC numbers between samples).
Stabilize RNA in whole blood
When collecting blood samples for RNA analysis, storage of the samples prior to RNA isolation is often required. The RiboPure™-Blood Kit contains RNAlater® Solution for the stabilization of RNA in whole blood. It also "freezes" the gene expression profile of the cells. Treated samples can be safely stored at ambient temperature for extended periods of time (up to three days or more). Blood samples stored in RNAlater® Solution yield RNA of comparable quality to blood samples processed directly (see figure).
Simple procedure yields highly pure RNA
The RiboPure™-Blood procedure consists of three steps: 1) addition of Lysis Solution directly to RNAlater®-treated or fresh whole blood, 2) initial RNA purification by phenol/chloroform extraction, and 3) final RNA purification on a glass fiber filter. An additional DNase treatment can then be performed with the DNA-free™ Reagents included in the kit. The entire procedure, including DNase treatment, can be completed in less than 1 hour.
Accessory products
The RNA can subsequently be enriched for white blood cell (WBC) mRNA using our GLOBINclear™ Kits to selectively remove globin transcripts. The Mouse RiboPure™-Blood RNA Isolation Kit (Cat. No. AM0951) is also available for the recovery of total RNA—and optionally microRNA—directly from mouse or rat blood. Mouse RiboPure™-Blood includes sample tubes preloaded with RNAlater® Solution to prevent RNA degradation and preserve RNA profiles.
WARNING: Cancer and Reproductive Harm – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Sample Type (General): | Blood |
|---|---|
| High Throughput Compatibility: | Not High Throughput-Compatible (Manual) |
| Downstream Application: | Microarray Analysis, Northern Blotting, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR) |
| Final Product: | Total RNA |
| Isolation Technology: | Organic Extraction, Spin Column |
| Product Line: | Ambion™, RiboPure™ |
| Product Size: | 40 preps |
| Starting Material (Amount): | Up to 0.5 ml whole blood |
| Yield (up to): | 4 µg |
Contents & storage
• 25 ml Elution Solution (4°C)
• 40 ml Lysis Solution (4°C)
• 25 ml Acid-Phenol:Chloroform (4°C)
• 5 ml Sodium Acetate Solution (4°C)
• 35 ml Wash Solution 1 (room temperature/4°C)
• 70 ml Wash 2/3 Concentrate (room temperature/4°C)
• 100 ml RNAlater® Solution (room temperature)
• 40 Filter Cartridges (room temperature)
• 80 Collecion Tubes (room temperature)
• 100 µl DNase I, 8 U/µl (-20°C, frost-free)
• 850 µl 20X DNase Buffer (-20°C)
• 1.2 ml DNase Inactivation Reagent (-20°C)
• 1 ml Formaldehyde Load Dye (-20°C)
This kit contains enough reagents for 40 RNA isolation from 300–500 µl whole blood.
• 40 ml Lysis Solution (4°C)
• 25 ml Acid-Phenol:Chloroform (4°C)
• 5 ml Sodium Acetate Solution (4°C)
• 35 ml Wash Solution 1 (room temperature/4°C)
• 70 ml Wash 2/3 Concentrate (room temperature/4°C)
• 100 ml RNAlater® Solution (room temperature)
• 40 Filter Cartridges (room temperature)
• 80 Collecion Tubes (room temperature)
• 100 µl DNase I, 8 U/µl (-20°C, frost-free)
• 850 µl 20X DNase Buffer (-20°C)
• 1.2 ml DNase Inactivation Reagent (-20°C)
• 1 ml Formaldehyde Load Dye (-20°C)
This kit contains enough reagents for 40 RNA isolation from 300–500 µl whole blood.
