Invitrogen™

RNAqueous™-Micro Total RNA Isolation Kit

Catalog number:  AM1931

Related applications:

DNA & RNA Purification & Analysis

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Description

The RNAqueous®-Micro Kit is used for phenol-free total RNA isolation from micro-sized samples (e.g., from <500,000 cultured cells, from few as 10 laser capture microdissected cells, or from ≤10 mg of tissue) using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. DNA-free™ DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR. This kit provides:

• Highly concentrated RNA—for increased sensitivity in downstream assays
• Isolation of high-quality RNA from micro-scale samples
• Reagents/protocol for RNA recovery from LCM samples

How it works
Samples are solubilized in a powerful chaotropic solution, and RNA is purified on a glass fiber filter cartridge specifically designed for very small samples. A simple modification of the protocol allows recovery of both large and small RNA species including tRNAs, 5S rRNA, and microRNAs. The RNA is eluted in a minimal volume of 20 µL. Residual genomic DNA is efficiently removed using the DNA-free™ system included in the kit. The RNAqueous®-Micro Kit contains enough reagents for 50 RNA isolations of DNA-free RNA.
WARNING: Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Final Product Type: Total RNA
For Use With (Application): Laser Capture Microdissection (LCM), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Purification Target: Total RNA
Sample Type: Cell Cultures, Laser Capture Microdissection (LCM), Cultured Cells
Isolation Technology: Silica Spin Column
Elution Volume Max: 20 µl
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Scale: Micro
Product Line: Ambion™, RNAqueous®

Contents & storage

• 50 Micro Filter Cartridge Assembly (room temperature)
• 50 Micro Elution Tubes (room temperature)
• 15 ml Wash Solution 1 Concentrate (room temperature)
• 28 ml Wash Solution 2/3 concentrate (4°C)
• 25 Lysis Solution (4 °C)
• 200 µl LCM Additive (-20°C)
• 55 µl DNase 2 U/µl (-20°C)
• 600 µl 10X DNase I Buffer (-20°C)
• 150 µl DNase Inactivation Reagent (-20°C)
• 5 ml Elution Solution (-20°C, 4°C, room temperature)