Invitrogen™

RNAqueous™-Micro Total RNA Isolation Kit

Catalog number: AM1931
Invitrogen™

RNAqueous™-Micro Total RNA Isolation Kit

Catalog number: AM1931
Catalog Number
AM1931
Unit Size
50 preps
Price (USD)
414.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
AM193150 preps
414.00
Your Price:
***
Product Overview
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The RNAqueous®-Micro Kit is used for phenol-free total RNA isolation from micro-sized samples (e.g., from <500,000 cultured cells, from few as 10 laser capture microdissected cells, or from ≤10 mg of tissue) using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. DNA-free™ DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR. This kit provides:

• Highly concentrated RNA—for increased sensitivity in downstream assays
• Isolation of high-quality RNA from micro-scale samples
• Reagents/protocol for RNA recovery from LCM samples

How it works
Samples are solubilized in a powerful chaotropic solution, and RNA is purified on a glass fiber filter cartridge specifically designed for very small samples. A simple modification of the protocol allows recovery of both large and small RNA species including tRNAs, 5S rRNA, and microRNAs. The RNA is eluted in a minimal volume of 20 µL. Residual genomic DNA is efficiently removed using the DNA-free™ system included in the kit. The RNAqueous®-Micro Kit contains enough reagents for 50 RNA isolations of DNA-free RNA.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format
Filter Cartridges
Elution Volume Max
20 µl
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Product Line
Ambion™, RNAqueous™
For Use With (Application)
Laser Capture Microdissection (LCM), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Final Product Type
Total RNA
Purification Target
Total RNA
Sample Type
Cell Cultures, Laser Capture Microdissection (LCM), Cultured Cells
No. of Reactions
50 Prep
Isolation Technology
Silica Spin Column
Scale
Micro

Contents & Storage

• 50 Micro Filter Cartridge Assembly (room temperature)
• 50 Micro Elution Tubes (room temperature)
• 15 ml Wash Solution 1 Concentrate (room temperature)
• 28 ml Wash Solution 2/3 concentrate (4°C)
• 25 Lysis Solution (4 °C)
• 200 µl LCM Additive (-20°C)
• 55 µl DNase 2 U/µl (-20°C)
• 600 µl 10X DNase I Buffer (-20°C)
• 150 µl DNase Inactivation Reagent (-20°C)
• 5 ml Elution Solution (-20°C, 4°C, room temperature)

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