LeukoLOCK™ Fractionation & Stabilization Kit

Catalog number: AM1933

Ambion™  Related applications: RNA Extraction

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Description

The LeukoLOCK™ Fractionation & Stabilization Kit is part of the LeukoLOCK™ Total RNA Isolation System, an innovative method for cellular fractionation of whole blood and total RNA stabilization and extraction from the leukocyte population. The LeukoLOCK™ system is comprised of a Fractionation & Stabilization kit (available separately here) and a LeukoLOCK™ Total RNA Isolation Kit, and is optimized for use with human blood. Blood is a storehouse of cellular information; however, the presence of globin mRNA in RNA prepared from whole blood can interfere with downstream expression profiling applications. The LeukoLOCK™ system employs filter-based leukocyte-depletion technology to isolate leukocytes from whole blood and RNAlater® Solution to stabilize the cells on the filter. By excluding red blood cells, the RNA that is purified from captured leukocytes is inherently depleted of globin mRNA, which improves sample utility for expression profiling and other applications (see Figure).

LeukoLOCK™ Fractionation & Stabilization Kit
A 9–10 mL sample of anticoagulated blood is passed through a LeukoLOCK™ filter, which captures the total leukocyte population while eliminating red blood cells (including reticulocytes), platelets, and plasma. After rinsing with phosphate-buffered saline, the filter is flushed with RNAlater® Solutionto stabilize the RNA in the captured leukocytes (see protocol schematic). The RNA can be isolated immediately, or stabilized cells can be maintained for several days at room temperature or for longer periods at –20°C or –80°C (see Figure).
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): Blood
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Downstream Application: Reverse Transcriptase PCR (RT-PCR), Northern Blotting, Real-Time Quantitative PCR (qPCR), Microarray Analysis, Nuclease Protection Assays
Product Size: 20 preps
Final Product: Cells (Leukocytes)
Yield (up to): 20 µg
Isolation Technology: Magnetic Bead, Vacuum Filter Column
Starting Material (Amount): Up to 10 ml whole blood
Shipping Condition: Room Temperature

Contents & storage

The RNAlater® Solution, Rubber Septum Caps, White Slip Connectors, Transfer Spikes, LeukoLOCK™ Filters, and Elution Solution, should be stored at room temperature. 1X PBS may be stored at any temperature.

Documents

Manuals & protocols

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