The Ambion® NorthernMax® Kit contains a complete set of RNase-free reagents for running formaldehyde-based northern analysis. This kit is ideal for the investigator who wants to use familiar reagents while taking advantage of NorthernMax® Kit's high sensitivity. Sufficient reagents are provided to process 1000 cm2 membrane.
• Increase sensitivity up to 100x over standard hybridization protocols • Reduce hybridization to just 2 hours for many messages • Complete kit contains ULTRAhyb® Hybridization Buffer and gel and wash reagents for 10–20 gels • Compatible with RNA, DNA, or oligonucleotide probes; labeled isotopically or nonisotopically
The included ULTRAhyb® Ultrasensitive Hybridization Solution increases the sensitivity of RNA probes up to 20x and DNA probes up to 100x. Hybridization can be completed in just 2 hours for many messages. The NorthernMax® Kit is compatible with DNA, RNA, or oligo probes labeled isotopically or nonisotopically. The NorthernMax® Kit contains everything you need to conduct northerns. Positively charged nylon membranes must be purchased separately (see Accessory Products). An RNA sample and template for probe synthesis are also provided as controls for each kit.
Accessory Products: The BrightStar® -Plus Positively Charged Nylon Membranes (SKU# AM10100, AM10102, or AM10104) are optimized for use with the NorthernMax® Kit.
Positive Control RNA, pTRI-GAPDH Mouse, DECAtemplate §-actin-Mouse, and Formaldehyde Load Dye should all be stored at –20°C. ULTRAhyb™ Buffer, 10X Denaturing Gel Buffer, and 10X MOPS Gel Running Buffer should be stored at 4°C. Agarose-LE™, Transfer Buffer, Low Stringency Wash Solution #1, High Stringency Wash Solution #2, and RNaseZap™ should be stored at room temperature. Nuclease-free water may be stored at any temperature.