Invitrogen™

Mouse RiboPure™-Blood RNA Isolation Kit

Catalog number: AM1951
Invitrogen™

Mouse RiboPure™-Blood RNA Isolation Kit

Catalog number: AM1951
Catalog Number
AM1951
Unit Size
25 preps
Price (USD)
Price: 358.00
Your Price:
Availability
-
Quantity
Catalog Number
Unit Size
25 preps
Price (USD)
Price: 358.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
AM195125 preps
Price: 358.00
Your Price:
-
AM1951M25 preps
Price: 358.00
Your Price:
-
Product Overview
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FAQ
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Additional Information
Recommendations
Recommendations
For the purification of extremely high-quality total RNA (and optionally, microRNA) from mouse or rat blood in about 35 minutes. The Ambion™ kit includes sufficient reagents for 25 RNA purifications from whole blood samples ranging from 0.1–0.5 each.

• Includes RNAlater™, predispensed in 2 mL tubes, for RNA stabilization
• Resulting RNA is suitable for stringent downstream applications such as array analysis and qRT-PCR

Due to interference from the enzymatic inhibitors and anticoagulants present in blood samples, RNA purification from whole blood requires a stringent isolation method that yields high-quality RNA depleted of inhibiting agents and contaminants. The kit includes microfuge tubes preloaded with RNAlater™ Tissue Collection:RNA Stabilization Solution, an aqueous, nontoxic storage reagent that prevents RNA degradation and preserves RNA profiles within samples. Stabilized samples can be stored at room temperature for up to 3 days or at –20°C for long-term storage. The related Figures show the performance of RNA isolated with the Mouse RiboPure™-Blood Kit in real-time RT-PCR. The kit includes an alternative protocol for isolating total RNA that contains microRNA. Typically, 30–55 µg of total RNA (containing microRNA) is obtained from 0.5 mL mouse blood. Yields from rat blood are considerably more variable, generally 18–40 µg from 0.5 mL blood.
WARNING: Cancer and Reproductive Harm – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format
Filter Cartridge
Isolation Technology
Organic Extraction, Spin Column
Purification Time
35 min.
Species
Mouse
Sample Type
Blood
Final Product Type
Total RNA, micro RNA
High-throughput Compatibility
Not High-throughput Compatible (Manual)
For Use With (Application)
Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Includes
Acid–Phenol:Chloroform, 3M sodium acetate pH 5.5, wash solution 1 concentrate, wash solution 2/3 concentrate, 2mL tubes pre-loaded with RNAlater, lysis solution, filter cartridges, collection tubes, nuclease-free water and elution solution
Prep Scale
100 µl - 1 ml Blood Samples ( Medium-Scale)
Product Line
Ambion™, RiboPure™
Product Type
Kit
Shipping Condition
Room Temperature
Starting Material Amount
Up to 0.5 ml whole blood
Target
miRNA, Total RNA
Yield
40 µg

Contents & Storage

Acid–Phenol:Chloroform, 3M Sodium Acetate pH 5.5, Wash Solution 1 Concentrate, Wash Solution 2⁄3 Concentrate at 4°C. Store 2 mL tubes pre–loaded with RNAlater, Lysis Solution, Filter Cartridges, Collection Tubes, Nuclease–free Water and Elution solution at room temperature.