Ambion® Poly(A) Polymerase catalyzes the addition of adenosine to the 3' end of RNA in a sequence-independent fashion. The enzyme can be used to append radiolabeled ATP to the 3' end of RNA molecules to generate labeled RNAs. One tube containing 80 U (2 U/ µL), 5X Poly(A) Polymerase Buffer, and 25 mM MnCl2 are provided. The enzyme can be used in conjunction with terminal transferase, reverse transcriptase, and thermostable polymerase to clone RNAs of unknown sequence.
Unit Definition: One unit is the amount of enzyme required to incorporate 1 nmol of AMP into acid-insoluble material in 10 min at 37°C.
For Research Use Only. Not for use in diagnostic procedures.
Poly(A) RNA Polymerases
Contents & storage
Poly(A) Polymerase, 5X Poly(A) Polymerase Buffer, and 25 mM MnCl2 should be stored at –20°C.