Invitrogen™

RNase Cocktail™ Enzyme Mix

Catalog number: AM2288
Invitrogen™

RNase Cocktail™ Enzyme Mix

Catalog number: AM2288
Catalog Number
AM2288
Unit Size
5 x 1 mL
Price (USD)
Price: 343.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
1 mL
Price (USD)
Price: 115.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
AM22885 x 1 mL
Price: 343.00
Your Price:
***
AM22861 mL
Price: 115.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
Recommendations
Ambion™ RNase Cocktail™ is a mixture of two highly purified ribonucleases, RNase A (500 U⁄ml) and RNase T1 (20,000 U⁄ml) and is free of DNase and nicking activities. Use RNase Cocktail for all situations where it is desirable to degrade RNA, i.e. plasmid minipreps and ribonuclease protection assays. RNase Cocktail is supplied in 50% glycerol for maximum convenience. Digestion of RNA with RNase A alone leaves fragments of RNA which are large enough to be visible on agarose gels and precipitate in ethanol. RNase A cuts after C and U residues, and RNase T1 cuts after G residues. Consequently, the mixture of both enzymes results in a reduction in RNA fragment size over the use of either alone.

Quality Control
RNase Cocktail Enzyme Mix is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and protease activity. Functionality is determined in a ribonuclease protection assay.

Unit Definitions:
RNase A: One unit of RNase A is the amount required to give an increase in absorption at 286 nm of 0.0146 absorbance units per minute in a 1 mL volume. Unit assay conditions: 100 mM Tris-acetate (pH 6.5), 1 mM EDTA and 1 mM cyclic 2', 3'-CMP.

RNase T1: 100 Units of RNase T1 is the amount of enzyme that yields an increase in absorption at 260 nm of 0.01428 units per min at room temperature using 60 µg⁄mL yeast total RNA as a substrate.

We also now offer Ambion™ RNA-Grade ribonucleases A, V1, and T1 for use in RNA structure⁄function studies. For more information, see: AM2274 RNase A (1 µg⁄ml), AM2275 RNase V1 (0.1 U⁄µl), AM2283 RNase T1 (1 U⁄µl).
WARNING: Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
500 U/mL (RNase A), 20,000 U/mL (RNase T1)
Shipping Condition
Dry Ice
Enzyme
RNase
Quantity
5 x 1 mL
Product Type
RNAse Cocktail Enzyme Mix
Product Line
Ambion™

Contents & Storage

-20°C

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