Invitrogen™

Proteinase K Solution (20 mg/mL)

Catalog number:  AM2546

Related applications:

Chromatin Biology

| DNA Extraction

| RNA Extraction

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Description

Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins. Proteinase K remains active:

• Over a wide pH range (optimal activity between 6.5 and 9.5)
• Under denaturing conditions (e.g., in the presence of SDS or urea)
• In the presence of metal chelating agents (e.g., EDTA)
• At comparatively high temperatures (optimum digestion temperature is 65°C)

Applications
Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.

Performance and quality testing
Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.

Unit definition
One mAnson unit is described as that amount of enzyme that liberates 1 µmole of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Liquid
Product Line: Ambion™
Product Size: 1.25 mL
Shipping Condition: Dry Ice
Species: T. album

Contents & storage

Store at –20°C.