Invitrogen™

SUPERase•In™ RNase Inhibitor (20 U/μL)

Catalog number:  AM2694

Related applications:

In Vitro Transcription

| Real Time PCR (qPCR)

| Reverse Transcription

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Description

SUPERase•In RNase Inhibitor is a protein-based inhibitor of non-human origin that noncovalently binds and inhibits the most common and troublesome RNases, including RNase A, B, C, 1, and T1.

SUPERase•In RNase Inhibitor can be used in any application where RNase contamination could be problematic. Because it inhibits a broader range of RNases than traditional RNase inhibitors, SUPERase•In is the most effective RNase inhibitor available, providing a higher level of protection against degradation.

SUPERase•In does not interfere with other enzymes such as RNA polymerases, reverse transcriptase, or Taq DNA polymerase. Additionally, SUPERase•In is active up to 65°C and over a pH range of 5.5 to 8.5.

If you are plannning to use this product with standard antibody purification methods, please contact our technical support to discuss experimental design.

Unit definition
SUPERase•In RNase Inhibitor at 1 U/µL will block the degradation of 0.1 µg/µL labeled RNA by 2.5 pg/µL of RNase A, 2.5 pg/µL of RNase I, and 0.0075 U/µL of RNase T1, for 4 hours at 37°C, in 20 mM Tris-HCl, pH 7.5, 50 mM NaCl, 1 mM EDTA. Analysis is by denaturing PAGE. SUPERase•In is currently the only ribonuclease inhibitor for which the unit activity is defined by such a functional assay.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Line: Ambion™,SUPERase•In™
Product Size: 2,500 units
Shipping Condition: Dry Ice

Contents & storage

Supplied in a single tube that should be stored at -20°C.

Storage buffer: 2 mM KH2PO4, 8 mM Na2HPO4, 2.7 mM KCl, 137 mM NaCl, pH 7.4 in 50% glycerol