Invitrogen™

Silencer™ Cy™3-labeled GAPDH siRNA (human, mouse, rat)

Catalog number: AM4649
Invitrogen™

Silencer™ Cy™3-labeled GAPDH siRNA (human, mouse, rat)

Catalog number: AM4649
Catalog Number
AM4649
Unit Size
5 nmol
Price (USD)
Price: 274.00
Online price: 264.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
AM46495 nmol
Price: 274.00
Online price: 264.65
Your price:
-
Product Overview
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Ambion™ fluorescently labeled control siRNA for the easy monitoring of siRNA uptake by fluorescence microscopy along with siRNA-induced knockdown. Purified, annealed, and ready to use, the 5 nmol aliquot is supplied in a stable, dried format.

• Fluorescently labeled siRNAs simplify the monitoring of siRNA delivery
• Ideal for correlating siRNA uptake with mRNA knockdown
• Functionally tested in several common cell lines
• HPLC purified, duplexed, and ready to use

Visualize siRNA Uptake with Labeled siRNAs
Fluorescent labeling of siRNAs allows direct observation of their cellular uptake, distribution, and localization. Fluorescently labeled siRNAs are commonly used to monitor transfection efficiency during optimization of transfection conditions. In general, siRNAs are rapidly taken up by and distributed throughout cells in as little as 4 hours.

Correlate siRNA Uptake with Gene Silencing
Studies show that the silencing activity of Silencer™ Cy™3 Labeled GAPDH siRNA is not affected by the fluorescent label. Therefore, these siRNAs are ideal for examining both siRNA uptake and siRNA-induced knockdown, and for correlating gene knockdown with transfection efficiency; this is important to know because different transfection reagents interact with siRNAs in different ways (i.e., some reagents may sequester the siRNA, resulting in poor gene knockdown). The Silencer™ Cy™3 Labeled GAPDH siRNA has the fluorescein derivative Cy™3 (excitation max. = 547, emission max. = 563 nm) on the 5' end of the sense strand, making it highly fluorescent when excited by the appropriate wavelength of light. Silencer™ Cy™3 Labeled GAPDH siRNA is suitable for monitoring siRNA uptake by chemical transfection or electroporation. Cy™3 labeling is recommended over FAM™ labeling when using electroporation, because of its brighter fluorescence signal visible over a broader pH range. Electroporation produces a more diffuse siRNA distribution pattern, resulting in a less intense fluorescence signal than with chemical transfection.

Accessory Products:
Silencer™ Cy™3 Labeled GAPDH siRNA (human) (SKU #AM4623) is also available as Silencer™ FAM™ Labeled GAPDH siRNA for human (SKU #AM4622) and for human, mouse, and rat (SKU #AM4650). Silencer™ control siRNAs are unmodified 21-mers; they are designed to be used with Silencer™ siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs. If you are using other siRNA products, such as Ambion™ Silencer™ Select siRNAs or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

RNAi Type
siRNA
Species
Human, Mouse, Rat
Label or Dye
Cy3
Product Line
Silencer™, Ambion™
Product Type
siRNA
Purity
HPLC
Quantity
5 nmol
Shipping Condition
Dry Ice
Target
GAPDH

Contents & Storage

The siRNA is provided dried down in a single tube, along with Nuclease-free Water for resuspension, and should be stored at –20°C.

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