Invitrogen™

Silencer™ Cy™3-labeled GAPDH siRNA (human, mouse, rat)

카탈로그 번호: AM4649
Invitrogen™

Silencer™ Cy™3-labeled GAPDH siRNA (human, mouse, rat)

카탈로그 번호: AM4649
카탈로그 번호
AM4649
제품 사이즈
5 nmol
제품 정가(KRW)
제품 정가 430,000
공급가
재고 정보
-
수량
카탈로그 번호제품 사이즈제품 정가(KRW)재고 정보수량
AM46495 nmol
제품 정가 430,000
공급가
-
제품 개요
그림
동영상
관련 제품 추천
관련 제품 추천
문서
FAQ
인용 및 참조 문헌
추가 정보
관련 제품 추천
Ambion™ fluorescently labeled control siRNA for the easy monitoring of siRNA uptake by fluorescence microscopy along with siRNA-induced knockdown. Purified, annealed, and ready to use, the 5 nmol aliquot is supplied in a stable, dried format.

• Fluorescently labeled siRNAs simplify the monitoring of siRNA delivery
• Ideal for correlating siRNA uptake with mRNA knockdown
• Functionally tested in several common cell lines
• HPLC purified, duplexed, and ready to use

Visualize siRNA Uptake with Labeled siRNAs
Fluorescent labeling of siRNAs allows direct observation of their cellular uptake, distribution, and localization. Fluorescently labeled siRNAs are commonly used to monitor transfection efficiency during optimization of transfection conditions. In general, siRNAs are rapidly taken up by and distributed throughout cells in as little as 4 hours.

Correlate siRNA Uptake with Gene Silencing
Studies show that the silencing activity of Silencer™ Cy™3 Labeled GAPDH siRNA is not affected by the fluorescent label. Therefore, these siRNAs are ideal for examining both siRNA uptake and siRNA-induced knockdown, and for correlating gene knockdown with transfection efficiency; this is important to know because different transfection reagents interact with siRNAs in different ways (i.e., some reagents may sequester the siRNA, resulting in poor gene knockdown). The Silencer™ Cy™3 Labeled GAPDH siRNA has the fluorescein derivative Cy™3 (excitation max. = 547, emission max. = 563 nm) on the 5' end of the sense strand, making it highly fluorescent when excited by the appropriate wavelength of light. Silencer™ Cy™3 Labeled GAPDH siRNA is suitable for monitoring siRNA uptake by chemical transfection or electroporation. Cy™3 labeling is recommended over FAM™ labeling when using electroporation, because of its brighter fluorescence signal visible over a broader pH range. Electroporation produces a more diffuse siRNA distribution pattern, resulting in a less intense fluorescence signal than with chemical transfection.

Accessory Products:
Silencer™ Cy™3 Labeled GAPDH siRNA (human) (SKU #AM4623) is also available as Silencer™ FAM™ Labeled GAPDH siRNA for human (SKU #AM4622) and for human, mouse, and rat (SKU #AM4650). Silencer™ control siRNAs are unmodified 21-mers; they are designed to be used with Silencer™ siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs. If you are using other siRNA products, such as Ambion™ Silencer™ Select siRNAs or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.
For Research Use Only. Not for use in diagnostic procedures.

사양

RNAi Type
siRNA
Human, Mouse, Rat
라벨 또는 염료
Cy3
제품라인
Silencer™, Ambion™
제품 유형
siRNA
순도
HPLC
수량
5 nmol
배송 조건
Dry Ice
Target
GAPDH

구성 및 보관

The siRNA is provided dried down in a single tube, along with Nuclease-free Water for resuspension, and should be stored at –20°C.

그림

문서 및 다운로드

Certificates | 증명서

    자주 묻는 질문(FAQ)

    인용 및 참조 문헌

    Search citations by name, author, journal title or abstract text