Invitrogen™

Random Decamers (50 µM)

Catalog number: AM5722G
Invitrogen™

Random Decamers (50 µM)

Catalog number: AM5722G
Catalog Number
AM5722G
Unit Size
80 µL
Price (USD)
132.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
AM5722G80 µL
132.00
Your Price:
***
Product Overview
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Random Decamers are the same primers currently included in the RETROscript® Kit (Cat. No. AM1710). They are provided at a stock concentration of 50 µM, and are functionally tested using the RETROscript® Kit.

Using random primers
Generally, reverse transcription reactions are primed with one of the following types of primers: random sequence oligonucleotides, oligo(dT), or an oligonucleotide that can hybridize with the specific RNA under study. The type of first-strand primer used for reverse transcription is mostly based on user preference. For cDNA library construction or cDNA labeling applications, oligo(dT) is almost always used to prime cDNA synthesis, so that the cDNA will start at the poly(A) tail. The greatest yield of RT product is usually obtained by using short random oligonucleotides to prime the reverse transcription, but this yield advantage may not be seen after the PCR step. When template is limiting, final RT-PCR yield may be somewhat higher when the reverse transcription is primed with random primers as opposed to oligo(dT) or gene-specific primers.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Line
Ambion™
Product Type
Primer
Shipping Condition
Dry Ice
Concentration
50 μm
Primer
Random

Contents & Storage

Store at –20°C.

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