Invitrogen™

Decade™ Markers System (to make radiolabeled RNA markers)

Catalog number:  AM7778

Related applications:

Nucleic Acid Gel Electrophoresis & Blotting

Back to top

Description

The Ambion® Decade™ Marker System produces a set of radiolabeled RNA molecules of 150, 100, 90, 80, 70, 60, 50, 40, 30, 20, and 10 nucleotides. The molecular weight markers are ideally suited for experiments where RNA molecules less than 150 nucleotides in length are being studied. Sufficient reagents are provided for 10 marker preparation reactions. This marker set is particularly useful for research laboratories studying tRNA, snRNA, snoRNA, miRNA, siRNA and other small RNA molecules. The system includes all of the reagents necessary to produce the molecular weight markers except gamma-32P-ATP. The Decade™ Markers derive from a single, gel-purified transcript that is provided. The transcript is 5' end-labeled using the kinase and kinase buffer supplied with the kit. The kinase reaction is then diluted into a cleavage reagent that generates the molecular weight marker set in a five minute, room temperature reaction. Gel loading buffer from the kit is then added and the marker is ready for electrophoresis. The labeling, cleavage, and gel preparation steps can be completed in less than an hour.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Gel Compatibility: Denaturing Polyacrylamide Gels
Product Line: Ambion™, Decade™
Ready to Load: No
Shipping Condition: Dry Ice
Size Range: 10 to 150 bp
Concentration: 100 μg/ml

Contents & storage

Decade™ Marker RNA should be stored at below -70°C. Polynucleotide Kinase, Kinase Reaction Buffer, Cleavage Reagent, Gel Loading Buffer II are stored at 4°C or -20°C. Nuclease-free water is stored at any temperature.