Invitrogen™

Decade™ Markers System (to make radiolabeled RNA markers)

Catalog number: AM7778
Invitrogen™

Decade™ Markers System (to make radiolabeled RNA markers)

Catalog number: AM7778
Catalog Number
AM7778
Unit Size
10 reactions
Price (USD)
218.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
AM777810 reactions
218.00
Your Price:
***
Product Overview
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The Ambion® Decade™ Marker System produces a set of radiolabeled RNA molecules of 150, 100, 90, 80, 70, 60, 50, 40, 30, 20, and 10 nucleotides. The molecular weight markers are ideally suited for experiments where RNA molecules less than 150 nucleotides in length are being studied. Sufficient reagents are provided for 10 marker preparation reactions. This marker set is particularly useful for research laboratories studying tRNA, snRNA, snoRNA, miRNA, siRNA and other small RNA molecules. The system includes all of the reagents necessary to produce the molecular weight markers except gamma-32P-ATP. The Decade™ Markers derive from a single, gel-purified transcript that is provided. The transcript is 5' end-labeled using the kinase and kinase buffer supplied with the kit. The kinase reaction is then diluted into a cleavage reagent that generates the molecular weight marker set in a five minute, room temperature reaction. Gel loading buffer from the kit is then added and the marker is ready for electrophoresis. The labeling, cleavage, and gel preparation steps can be completed in less than an hour.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration
100 μg/ml
Gel Type
Polyacrylamide
Gel Compatibility
Denaturing Polyacrylamide Gels
Shipping Condition
Dry Ice
Product Line
Ambion™, Decade™
Product Type Specs
Markers System
Ready to Load
No
Size Range
10 to 150 bp

Contents & Storage

Decade™ Marker RNA should be stored at below -70°C. Polynucleotide Kinase, Kinase Reaction Buffer, Cleavage Reagent, Gel Loading Buffer II are stored at 4°C or -20°C. Nuclease-free water is stored at any temperature.

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