When incorporated into DNA, 5-(3-aminoallyl)-dUTP provides a reactive group for addition of other chemical groups. The modified dUTP is readily incorporated by reverse transcriptase. The aminoallyl modification enables downstream reaction with amine-reactive compounds such as N-hydroxysuccinimide (NHS) esters; thus aminoallyl-modified cDNA can be labeled with any amine-reactive group moiety.
Use of this Modified Nucleotide: Use 5-(3-aminoallyl)-dUTP as recommended in your protocol for reverse transcription. Substitution of 30–80% of the dTTP with 5-(3-aminoallyl)-dUTP may be useful, but the exact proportion will depend on experimental goals.
For Research Use Only. Not for use in diagnostic procedures.
Contents & storage
Store at or below -70°C. Avoid multiple freeze-thaw cycles. Aliquots of the product may be stored short-term at -20°C. Do not store in a frost-free freezer.