Ambion™ Biotinylated UTPs are ideal for use as substrates in in vitro transcription reactions. These modified nucleotides can be utilized by a variety of RNA polymerases, including T7, T3, and SP6 RNA polymerases, as well as other RNA modifying enzymes. It is provided in one tube containing 25 μL (250 nmol). Biotinylated RNA can be used in place of radioactively labeled RNA in many applications, including cRNA amplification and labeling for microarray target preparation. Detection is then carried out via one of a variety of streptavidin-based methods. This Bio-UTP is confirmed free of non-specific endonuclease activity, exonuclease activity, and RNase activity and is functionally tested using the MessageAmp™ II aRNA Amplification Kit (SKU# AM1751).
For Research Use Only. Not for use in diagnostic procedures.