EVOS™ FL Auto 2 Imaging System

Catalog number:  AMAFD2000

 Related applications:

Cellular Imaging

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The EVOS™ FL Auto 2 Imaging System is a high-performance fully-automated inverted multi-channel fluorescence and transmitted light imaging system. It was developed to meet today’s expectations of user interface interactions, data generation speed and flexibility, and end-user value, without compromising image quality. The powerful and time-saving acquisition tools with enhanced autofocus algorithms and automated routines for microwell plate assays, time-lapse live cell imaging, area scanning in time lapse and Z-stacks in scan mode, and a superb optomechanical design allow you to generate publication quality images and data in seconds, when and where needed.

The EVOS FL Auto 2 Imaging System offers you these important advantages:

• Outstanding image quality and versatility with a 5-position objective turret, 4-color fluorescence and transmitted light channels, plus color and B/W cameras
• Exceptional usability with fully automated X/Y scanning stage, autofocus, and acquisition routines
• High-speed image acquisition coupled with multi-position well scanning. Z-stack and tile-stitch options lend power and flexibility to your data generation.
• Fully integrated time-lapse live cell imaging using the optional EVOS Onstage Incubator for precise control of temperature, gases for normoxic or hypoxic conditions and humidity
• Powerful image analysis capabilities for cell segmentation and quantification with the optional Celleste™ Image Analysis Software

Fully automated imaging system
The EVOS FL Auto 2 system combines and integrates precision components with a unique modern design functionality that enables high-quality automated fluorescence imaging anywhere with unprecedented work-flow efficiency. Full automation of the precision X/Y-stage movement; changing of the 4 LED fluorescence light cubes, 5-position objective turret, focus, and exposure; and dual camera switching make the EVOS FL Auto 2 system an exceptional platform for a variety of demanding imaging applications. Through the intuitive acquisition interface, you can program the EVOS FL Auto 2 system to run well-plate scanning, time lapse experiments, and tile-stitch/montage area scans—in Z-stack and/or time-lapse modes—in your vessel of choice. For live cell imaging over multiple hours or days, the optional EVOS Onstage Incubator, which functions as an environmental chamber on top of the microscope stage, is operated seamlessly from within the instrument interface. EVOS FL Auto 2 system is compatible with all accessories for the predecessor EVOS FL Auto imaging system.

Versatile and highly configurable
With a 5-position objective turret and the ability to simultaneous acquire images in 4 fluorescence channels plus transmitted light, the optical system can be easily configured to meet your needs. Choose from our broad range of high quality coverslip-corrected and long working distance objectives for use with plastic vessels or glass sides. Available from 1.25X to 100X magnification in achromat, fluorite, or apochromat models, together with over twenty LED light cubes, the sample imaging options are limitless. Dual monochrome and color high-sensitivity dual CMOS cameras enable seamless imaging of both fluorescent and chromogenically stained samples. Interchangeable vessel holders accommodate most vessel types and sizes, including slides, multi-well plates, culture flasks, and Petri dishes, and afford precise control and sample alignment by the automated stage. The vessel creation wizard in the user interface enables creation of profiles for custom sample vessels.

Powerful software
The EVOS FL Auto 2 software has been completely rewritten, presenting users with a new experience and many new tools for acquisition, visualization, and analysis. The user interface was designed with a strong emphasis on usability, in keeping with a long-standing EVOS principle. Examples include a novel concept of giving users an option of viewing samples in a single field mode (Field View) and also a window that covers a much larger area (Area View). By using a zoom function, the Area View size can be adjusted. A user can, for example, explore a large sample like a tissue slice, define regions of interest, and then seamlessly transition to the Scan function in Automate Mode and execute the scan, at higher magnification if desired. The enhanced power of the EVOS FL Auto 2 system enables more demanding automated acquisition routines like area scanning in time lapse and Z-stacks in scan mode to be performed. New visualization capabilities include multi-field image viewing gallery, mosaic tiling and tile stitching options, contrast stretching, Z-stack visualization, a video recording and a movie maker/editor for time lapse sequences. EVOS FL Auto 2 system is fully compatible with the EVOS Onstage Incubator environmental chamber for control of temperature, gases, and humidity. With the ability generate both hypoxic and normoxic conditions it is possible to study live cells over extended periods with highly flexible time lapse automation routines.

For users needing more powerful image analysis capabilities, images acquired on the EVOS FL Auto 2 system can be analyzed directly using the optional Celleste Image Analysis Software. Celleste software offers powerful tools for image segmentation and classification that can be used for cell counting; measuring intensity, area, and shape changes over time; and more.

Smart LED illumination technology
All EVOS fluorescence imaging systems utilize our proprietary LED light cubes. This light engine outputs remarkable intensity over a short light-path and delivers incomparable fluorophore excitation. Each light cube contains a precisely matched set of optical components to optimize the position, evenness, and intensity of the light beam. Digitally controlled LED light sources allow adjustment of illumination levels to the sample and experimental conditions to minimize the risk of photo-toxicity and photo-bleaching in time-lapse studies. Hard-coated filters give sharper edges and significantly higher transmission efficiencies than traditional soft-coated filters.

Easy to use and reliable
The EVOS FL Auto 2 Imaging System is powerful, yet offers the simplicity of plug-and-play. Like other EVOS systems, it requires no warm-up or cool-down periods. The LED light source guarantees exceptional stability and durability—so you can turn the unit on and off whenever you need to image a sample. The environmentally safe, mercury-free LED bulbs are rated for >50,000 hours, compared to 300 hours for a typical mercury bulb and 1,500 hours for a metal halide bulb. The long lifetime and low energy consumption translate into significantly lower operating costs compared to instruments with conventional light sources. The EVOS FL Auto 2 system is powered by an associated PC with 16 GB RAM. Whether acquiring single images or setting up automated routines, the EVOS FL Auto 2 system is remarkably easy to use and run. The 1920 x 1080 pixel resolution 23" LCD touch screen monitor enables “finger swipes” to expand or zoom in on images, and moving between microwell plate wells is done by a single touch. Operation is also also fully controllable via mouse.

The EVOS imaging systems are built from the ground up to maximize performance and optimize workflow. You will be astonished at how easy it is to operate and amazed at how extraordinary your images look on-screen.

More information about the EVOS FL Auto 2 Imaging System and software
Explore the entire EVOS line of imaging systems, accessories, and offers

System & hardware

Optics: Infinity‐corrected optical system; RMS‐threaded objectives with 45 mm parfocal distance
Illumination: Adjustable intensity LED (>50,000‐hour life per light cube); broad selection of patented standard and specialty light cubes
Light cubes (not included): Commonly used light cubes include: DAPI (Ex/Em: 360/447 nm), GFP (470/525 nm), RFP (530/593 nm), Texas Red (585/624), Cy5 (628/692 nm)
Contrast methods: Fluorescence and transmitted light (brightfield & phase contrast)
Objective turret: 5‐position; front‐mounted control
Objectives (not included): Wide selection of high‐quality LWD and coverslip‐corrected objectives
Condenser: 60-mm long working distance condenser; 4-position turret with a clear aperture and 3 phase annuli
Stage: Motorized X/Y scanning stage; travel range 120 mm x 80 mm with sub‐micron resolution, drop‐in inserts to receive vessel holders, and lock down holders to fix sample in place during long scans
Focus mechanism: Automated focus mechanism with sub‐micron resolution
LCD display: 23" high‐resolution touch screen color monitor (also fully controllable via mouse); 1920 x 1080 pixel resolution
Cameras: High-sensitivity 1.3 MP CMOS monochrome camera with 1328 x 1048 pixels,high-sensitivity 3.2 MP CMOS color camera with 2080 x 1552 pixels
Computer: External PC with 16 GB RAM running Windows® 7 Pro designed to operate with touchscreen monitor and instrument
Captured images: 16‐bit monochrome TIFF or PNG (12‐bit dynamic range); 24‐bit color TIFF, PNG JPG, BMP; time‐lapse AVI
Output ports: Microscope: 2 USB 3.0, 1 USB 2.0, Power
Computer: Multiple USB 3 ports, Display Port, HDMI, Ethernet
Networking capability: Connection through Windows/SMB network via an Ethernet cable connection
Power supply: AC adapter with country‐specific power cords
Dimensions (LxWxH): 18 x 14 x 13 inches,457 x 330 x 356 mm
Weight: 16/35 (kg/lb)

For Research Use Only. Not for use in diagnostic procedures.


Color: Visible Spectrum Colors
Detection Method: Brightfield, Color Brightfield, Fluorescent, Phase Contrast
For Use With (Equipment): EVOS™
Format: Fluidigm C1 Microfluidics Plate, Bioptechs Perfusion Dish, Fluxion™ BioFlux™ Heating Stage, Haemocytometer, IVF Dish, Multi-well plate formats from 6-well plate to 1,536-well plate, Petri dishes from 35mm to 100mm, Slide(s), T-flasks from 25mL (T-25) to 150mL (T-150), Terasaki Plate, Tokai Hit™ Heated Stage Plate
Product Line: EVOS™
Product Size: 1 system
Shipping Condition: Room Temperature

Contents & storage

• EVOS FL Auto 2 Imaging System
• 23" high‐resolution touch screen color monitor (also fully controllable via mouse); 1920 x 1080 pixel resolution
• External PC with 16 GB RAM running Windows® 7 Pro designed to operate with touchscreen monitor and instrument
• EVOS FL Auto 2 Accessory Kit
     --EVOS FL Auto 2 Quick Start Guide
     --16 GB USB 3.0 drive with user manual
     --2 North America (Type B) power cords for the microscope and PC*
     --1 North America (Type B) power cords for the monitor**
     --USB 2.0 type A to B cable
     --USB 3.0 type A to B cable (2 ea)
     --Display port to display port cable, 6 ft
     --Universal power supply, 24V, 5A
     --Cube Access Tool
     --EVOS Calibration Slide (AMEP4720)
     --Vessel holder with retention for two slides (AMEPVH021)
     --Vessel holder with retention for microwell plates (AMEPVH022). Also functions as adapter for vessel holders AMEPVH001 through AMEPVH018.
     --Block Condenser Slider (AMEP4688)
     --Diffuser Condenser Slider for brightfield applications (AMEPDFS1)
     --Slider for 4X objectives (AMEP4738)
     --EVOS Light Shield
     --EVOS Dust Cover
     --Shipping Restraint Removal Guide
     --Mouse Pad
     --Tech Support card

*Note that three country-specific power cords for the microscope and PC must be ordered separately in regions not using the North America Type B power plug:
• EVOS Power Cord, Type G (United Kingdom), Cat. No. AMEP4645
• EVOS Power Cord, Type C (Europe), Cat. No. AMEP4646
• EVOS Power Cord, Type I (Australia), Cat. No. AMEP4647
• EVOS Power Cord, Type L (Italy), Cat. No. AMEP4648
• EVOS Power Cord, Type H (Israel), Cat. No. AMEP4649
• EVOS Power Cord, Type D (India/South Africa), Cat. No. AMEP4708


Manuals & protocols